Determining the fatty acid composition and antioxidant activities of Centaurea virgata Lam.

Burcu Sümer Tüzün; Tuğçe Fafal; Bijen Kıvçak

Makale detayları

Determining the fatty acid composition and antioxidant activities of Centaurea virgata Lam.

Yazarlar:

Burcu Sümer Tüzün | Tuğçe Fafal | Bijen Kıvçak

Yayıncı:

Istanbul University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences

Dergi:

İstanbul Journal of Pharmacy Vol.54 Issue 2, pp.182-188

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001329746

pISSN:

2587-2087

Anahtar kelimeler:

Özet:

 Background and Aims: Centaurea is a significant widespread genus in the Asteraceae family. Türkiye has 194 species, of which 118 are endemic. Free radicals can alter lipids, proteins, and DNA and trigger many diseases. However, a person with an insufficient immune system needs outside assistance to scavenge free radicals. Antioxidants protect the body from oxidative stress by scavenging free radicals. Fats are also very important because they contain fat-soluble vitamins, form lipoproteins with proteins, and affect health. Fatty acids serve as part of molecules in cells or alone as building blocks of cell membranes. They also promote energy and signaling molecules. Polyunsaturated fatty acids PUFAs have significant antioxidant activity. Studies are found showing PUFAs to be able to be used as a preservative in foods. These effects are due to phenolic hydroxyl groups. The aim of the current study is to evaluate the fatty acid profile of C. virgata to determine the fatty acid content and examine the antioxidant activities related to major fatty acids. Methods: Gas chromatography Mass Spectrometry GCMS analyses were carried out to determine the fatty acid content. Furthermore, the in vitro antioxidant activities of methanol extract were determined using DPPH 2, 2-Diphenyl-1-picrylhydrazyl and ABTS2,2-Azino-bis 3-ethylbenzothiazoline-6-sulfonic acid diammonium salt radical scavenging and the reducing power assay methods. The program IBM SPSS Statistics ver. 25 was used to evaluate antioxidant activities. Results: C. virgatas major components were found to be palmitic acid 30.13, oleic acid 9.17, and linoleic acid 11.64. The DPPH results indicated the IC50 value of the methanol extract to be 378.86  0.60 μgmL, while ABTS was found to be 19.38  0.49 μgmL. The results from the reducing power assay found the absorbance of ascorbic acid to be 0.55 at a 100 μgmL concentration and it measure 0.12 in C. virgata. Conclusion: According to the evaluation results, harmony is seen to exist between the groups regarding the activities.

 Background and Aims: Centaurea is a significant widespread genus in the Asteraceae family. Türkiye has 194 species, of which 118 are endemic. Free radicals can alter lipids, proteins, and DNA and trigger many diseases. However, a person with an insufficient immune system needs outside assistance to scavenge free radicals. Antioxidants protect the body from oxidative stress by scavenging free radicals. Fats are also very important because they contain fat-soluble vitamins, form lipoproteins with proteins, and affect health. Fatty acids serve as part of molecules in cells or alone as building blocks of cell membranes. They also promote energy and signaling molecules. Polyunsaturated fatty acids PUFAs have significant antioxidant activity. Studies are found showing PUFAs to be able to be used as a preservative in foods. These effects are due to phenolic hydroxyl groups. The aim of the current study is to evaluate the fatty acid profile of C. virgata to determine the fatty acid content and examine the antioxidant activities related to major fatty acids. Methods: Gas chromatography Mass Spectrometry GCMS analyses were carried out to determine the fatty acid content. Furthermore, the in vitro antioxidant activities of methanol extract were determined using DPPH 2, 2-Diphenyl-1-picrylhydrazyl and ABTS2,2-Azino-bis 3-ethylbenzothiazoline-6-sulfonic acid diammonium salt radical scavenging and the reducing power assay methods. The program IBM SPSS Statistics ver. 25 was used to evaluate antioxidant activities. Results: C. virgatas major components were found to be palmitic acid 30.13, oleic acid 9.17, and linoleic acid 11.64. The DPPH results indicated the IC50 value of the methanol extract to be 378.86  0.60 μgmL, while ABTS was found to be 19.38  0.49 μgmL. The results from the reducing power assay found the absorbance of ascorbic acid to be 0.55 at a 100 μgmL concentration and it measure 0.12 in C. virgata. Conclusion: According to the evaluation results, harmony is seen to exist between the groups regarding the activities.

 Background and Aims: Centaurea is a significant widespread genus in the Asteraceae family. Türkiye has 194 species, of which 118 are endemic. Free radicals can alter lipids, proteins, and DNA and trigger many diseases. However, a person with an insufficient immune system needs outside assistance to scavenge free radicals. Antioxidants protect the body from oxidative stress by scavenging free radicals. Fats are also very important because they contain fat-soluble vitamins, form lipoproteins with proteins, and affect health. Fatty acids serve as part of molecules in cells or alone as building blocks of cell membranes. They also promote energy and signaling molecules. Polyunsaturated fatty acids PUFAs have significant antioxidant activity. Studies are found showing PUFAs to be able to be used as a preservative in foods. These effects are due to phenolic hydroxyl groups. The aim of the current study is to evaluate the fatty acid profile of C. virgata to determine the fatty acid content and examine the antioxidant activities related to major fatty acids. Methods: Gas chromatography Mass Spectrometry GCMS analyses were carried out to determine the fatty acid content. Furthermore, the in vitro antioxidant activities of methanol extract were determined using DPPH 2, 2-Diphenyl-1-picrylhydrazyl and ABTS2,2-Azino-bis 3-ethylbenzothiazoline-6-sulfonic acid diammonium salt radical scavenging and the reducing power assay methods. The program IBM SPSS Statistics ver. 25 was used to evaluate antioxidant activities. Results: C. virgatas major components were found to be palmitic acid 30.13, oleic acid 9.17, and linoleic acid 11.64. The DPPH results indicated the IC50 value of the methanol extract to be 378.86  0.60 μgmL, while ABTS was found to be 19.38  0.49 μgmL. The results from the reducing power assay found the absorbance of ascorbic acid to be 0.55 at a 100 μgmL concentration and it measure 0.12 in C. virgata. Conclusion: According to the evaluation results, harmony is seen to exist between the groups regarding the activities.

 Background and Aims: Centaurea is a significant widespread genus in the Asteraceae family. Türkiye has 194 species, of which 118 are endemic. Free radicals can alter lipids, proteins, and DNA and trigger many diseases. However, a person with an insufficient immune system needs outside assistance to scavenge free radicals. Antioxidants protect the body from oxidative stress by scavenging free radicals. Fats are also very important because they contain fat-soluble vitamins, form lipoproteins with proteins, and affect health. Fatty acids serve as part of molecules in cells or alone as building blocks of cell membranes. They also promote energy and signaling molecules. Polyunsaturated fatty acids PUFAs have significant antioxidant activity. Studies are found showing PUFAs to be able to be used as a preservative in foods. These effects are due to phenolic hydroxyl groups. The aim of the current study is to evaluate the fatty acid profile of C. virgata to determine the fatty acid content and examine the antioxidant activities related to major fatty acids. Methods: Gas chromatography Mass Spectrometry GCMS analyses were carried out to determine the fatty acid content. Furthermore, the in vitro antioxidant activities of methanol extract were determined using DPPH 2, 2-Diphenyl-1-picrylhydrazyl and ABTS2,2-Azino-bis 3-ethylbenzothiazoline-6-sulfonic acid diammonium salt radical scavenging and the reducing power assay methods. The program IBM SPSS Statistics ver. 25 was used to evaluate antioxidant activities. Results: C. virgatas major components were found to be palmitic acid 30.13, oleic acid 9.17, and linoleic acid 11.64. The DPPH results indicated the IC50 value of the methanol extract to be 378.86  0.60 μgmL, while ABTS was found to be 19.38  0.49 μgmL. The results from the reducing power assay found the absorbance of ascorbic acid to be 0.55 at a 100 μgmL concentration and it measure 0.12 in C. virgata. Conclusion: According to the evaluation results, harmony is seen to exist between the groups regarding the activities.

Makale detayları

Determining the fatty acid composition and antioxidant activities of Centaurea virgata Lam.

Yazarlar:

Burcu Sümer Tüzün | Tuğçe Fafal | Bijen Kıvçak

Yayıncı:

Istanbul University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences

Dergi:

İstanbul Journal of Pharmacy Vol.54 Issue 2, pp.182-188

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001329746

pISSN:

2587-2087

ABTS

Centaurea

DPPH

Fatty acid

GC-MS

Sayı

Makale detayları

Determining the fatty acid composition and antioxidant activities of Centaurea virgata Lam.

Yazarlar: Burcu Sümer Tüzün | Tuğçe Fafal | Bijen Kıvçak

Yayıncı: Istanbul University

Ana Başlıklar: Pharmacology and Pharmaceutical Sciences

Dergi: İstanbul Journal of Pharmacy Vol.54 Issue 2, pp.182-188

Belge Tipi: Makale

Yayın Tarihi: 8/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001329746

pISSN: 2587-2087

ABTS

Centaurea

DPPH

Fatty acid

GC-MS

Sayı

Yazarlar:

Burcu Sümer Tüzün | Tuğçe Fafal | Bijen Kıvçak

Yayıncı:

Istanbul University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences

Dergi:

İstanbul Journal of Pharmacy Vol.54 Issue 2, pp.182-188

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001329746

pISSN:

2587-2087