Hyperbaric oxygen therapy mitigates cochlear damage induced by a high-fructose diet: a histopathological study in rats

Mehmet Akif Baktır; Esra Balcıoğlu

Makale detayları

Hyperbaric oxygen therapy mitigates cochlear damage induced by a high-fructose diet: a histopathological study in rats

Yazarlar:

Mehmet Akif Baktır | Esra Balcıoğlu

Yayıncı:

MediHealth Academy Yayıncılık

Ana Başlıklar:

Halk Sağlığı Beslenmesi | Sualtı Hekimliği ve Hiperbarik Tıp

Dergi:

Anatolian Current Medical Journal Vol.7 Issue 4, pp.518-523

Belge Tipi:

Makale

Yayın Tarihi:

7/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001355616

pISSN:

2718-0115

Anahtar kelimeler:

Özet:

 Aims: Hearing loss, an increasing worldwide issue, has been associated with increased carbohydrate consumption. This study investigates the histopathological impact of a high-fructose diet HFD on cochlear structures and assesses whether hyperbaric oxygen therapy HBOT can mitigate those effects. Methods: Adult male Wistar rats were divided into four groups n8 each: control standard diet, 10 weeks, HFD 60 fructose diet, 10 weeks, HBOT standard dietdaily HBOT at 2.4 ATA for 1 h during weeks 910, and HFDHBOT 60 fructose dietHBOT in weeks 910. After 10 weeks, cochleae were took out and stria vascularis thickness, basilar membrane thickness, and innerouter hair cell lengths were measured in basal, middle, and apical turns. Cochlear cross-sections stained with HE and Massons trichrome, and evaluated histopathologically under light microscope. Results: Compared to controls, HFD-fed rats had thinner stria vascularis and basilar membranes in basal and intermediate turns and shorter hair cell lengths throughout all turns. The apical turn outer hair cell length and the middle and apical turns inner hair cell length increased statistically in HFDHBOT rats compared to HFD alone. No significant changes were seen between HBOT-only and control groups. HFD induces considerable cochlear damage and cellular death in the organ of Corti in subjects. HBOT application somewhat mitigates these effects. Conclusion: A 10-week HFD induces region-specific cochlear structural degeneration in rats, and HBOT during the final two weeks partially reverses these changes. Future studies with larger sample sizes should include functional auditory assessments and explore underlying molecular mechanisms.

 Aims: Hearing loss, an increasing worldwide issue, has been associated with increased carbohydrate consumption. This study investigates the histopathological impact of a high-fructose diet HFD on cochlear structures and assesses whether hyperbaric oxygen therapy HBOT can mitigate those effects. Methods: Adult male Wistar rats were divided into four groups n8 each: control standard diet, 10 weeks, HFD 60 fructose diet, 10 weeks, HBOT standard dietdaily HBOT at 2.4 ATA for 1 h during weeks 910, and HFDHBOT 60 fructose dietHBOT in weeks 910. After 10 weeks, cochleae were took out and stria vascularis thickness, basilar membrane thickness, and innerouter hair cell lengths were measured in basal, middle, and apical turns. Cochlear cross-sections stained with HE and Massons trichrome, and evaluated histopathologically under light microscope. Results: Compared to controls, HFD-fed rats had thinner stria vascularis and basilar membranes in basal and intermediate turns and shorter hair cell lengths throughout all turns. The apical turn outer hair cell length and the middle and apical turns inner hair cell length increased statistically in HFDHBOT rats compared to HFD alone. No significant changes were seen between HBOT-only and control groups. HFD induces considerable cochlear damage and cellular death in the organ of Corti in subjects. HBOT application somewhat mitigates these effects. Conclusion: A 10-week HFD induces region-specific cochlear structural degeneration in rats, and HBOT during the final two weeks partially reverses these changes. Future studies with larger sample sizes should include functional auditory assessments and explore underlying molecular mechanisms.

 Aims: Hearing loss, an increasing worldwide issue, has been associated with increased carbohydrate consumption. This study investigates the histopathological impact of a high-fructose diet HFD on cochlear structures and assesses whether hyperbaric oxygen therapy HBOT can mitigate those effects. Methods: Adult male Wistar rats were divided into four groups n8 each: control standard diet, 10 weeks, HFD 60 fructose diet, 10 weeks, HBOT standard dietdaily HBOT at 2.4 ATA for 1 h during weeks 910, and HFDHBOT 60 fructose dietHBOT in weeks 910. After 10 weeks, cochleae were took out and stria vascularis thickness, basilar membrane thickness, and innerouter hair cell lengths were measured in basal, middle, and apical turns. Cochlear cross-sections stained with HE and Massons trichrome, and evaluated histopathologically under light microscope. Results: Compared to controls, HFD-fed rats had thinner stria vascularis and basilar membranes in basal and intermediate turns and shorter hair cell lengths throughout all turns. The apical turn outer hair cell length and the middle and apical turns inner hair cell length increased statistically in HFDHBOT rats compared to HFD alone. No significant changes were seen between HBOT-only and control groups. HFD induces considerable cochlear damage and cellular death in the organ of Corti in subjects. HBOT application somewhat mitigates these effects. Conclusion: A 10-week HFD induces region-specific cochlear structural degeneration in rats, and HBOT during the final two weeks partially reverses these changes. Future studies with larger sample sizes should include functional auditory assessments and explore underlying molecular mechanisms.

 Aims: Hearing loss, an increasing worldwide issue, has been associated with increased carbohydrate consumption. This study investigates the histopathological impact of a high-fructose diet HFD on cochlear structures and assesses whether hyperbaric oxygen therapy HBOT can mitigate those effects. Methods: Adult male Wistar rats were divided into four groups n8 each: control standard diet, 10 weeks, HFD 60 fructose diet, 10 weeks, HBOT standard dietdaily HBOT at 2.4 ATA for 1 h during weeks 910, and HFDHBOT 60 fructose dietHBOT in weeks 910. After 10 weeks, cochleae were took out and stria vascularis thickness, basilar membrane thickness, and innerouter hair cell lengths were measured in basal, middle, and apical turns. Cochlear cross-sections stained with HE and Massons trichrome, and evaluated histopathologically under light microscope. Results: Compared to controls, HFD-fed rats had thinner stria vascularis and basilar membranes in basal and intermediate turns and shorter hair cell lengths throughout all turns. The apical turn outer hair cell length and the middle and apical turns inner hair cell length increased statistically in HFDHBOT rats compared to HFD alone. No significant changes were seen between HBOT-only and control groups. HFD induces considerable cochlear damage and cellular death in the organ of Corti in subjects. HBOT application somewhat mitigates these effects. Conclusion: A 10-week HFD induces region-specific cochlear structural degeneration in rats, and HBOT during the final two weeks partially reverses these changes. Future studies with larger sample sizes should include functional auditory assessments and explore underlying molecular mechanisms.

Makale detayları

Hyperbaric oxygen therapy mitigates cochlear damage induced by a high-fructose diet: a histopathological study in rats

Yazarlar:

Mehmet Akif Baktır | Esra Balcıoğlu

Yayıncı:

MediHealth Academy Yayıncılık

Ana Başlıklar:

Halk Sağlığı Beslenmesi | Sualtı Hekimliği ve Hiperbarik Tıp

Dergi:

Anatolian Current Medical Journal Vol.7 Issue 4, pp.518-523

Belge Tipi:

Makale

Yayın Tarihi:

7/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001355616

pISSN:

2718-0115

Hyperbaric oxygen therapy

high-fructose diet

inner hair cell

organ of corti

outer hair cellHyperbaric oxygen therapy

high-fructose diet

inner hair cell

organ of corti

outer hair cel

Sayı

Makale detayları

Yazarlar: Mehmet Akif Baktır | Esra Balcıoğlu

Yayıncı: MediHealth Academy Yayıncılık

Ana Başlıklar: Halk Sağlığı Beslenmesi | Sualtı Hekimliği ve Hiperbarik Tıp

Dergi: Anatolian Current Medical Journal Vol.7 Issue 4, pp.518-523

Belge Tipi: Makale

Yayın Tarihi: 7/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001355616

pISSN: 2718-0115

Sayı

Yazarlar:

Mehmet Akif Baktır | Esra Balcıoğlu

Yayıncı:

MediHealth Academy Yayıncılık

Ana Başlıklar:

Halk Sağlığı Beslenmesi | Sualtı Hekimliği ve Hiperbarik Tıp

Dergi:

Anatolian Current Medical Journal Vol.7 Issue 4, pp.518-523

Belge Tipi:

Makale

Yayın Tarihi:

7/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001355616

pISSN:

2718-0115