The Comparison of Two Different Multiplex Respiratory PCR Panels and The Evaluation of the Viral and Bacterial Agents

Nurullah Çiftçi; Gizem Tükenmez; Murat Karameşe

Makale detayları

The Comparison of Two Different Multiplex Respiratory PCR Panels and The Evaluation of the Viral and Bacterial Agents

Yazarlar:

Nurullah Çiftçi | Gizem Tükenmez | Murat Karameşe

Yayıncı:

Dicle University

Ana Başlıklar:

Tıp Eğitimi | Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi:

Dicle Tıp Dergisi Vol.52 Issue 2, pp.273-281

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001348134

pISSN:

1300-2945

Anahtar kelimeler:

Özet:

 Objective: Respiratory tract infections RTIs are among the leading causes of morbidity and mortality in children worldwide. This study aimed to compare the diagnostic performance of two multiplex PCR MT-PCR panels, 7-pathogen and 24- pathogen, for detecting viral and bacterial pathogens in pediatric patients having upper respiratory tract infection URTI symptoms. Methods: The study was conducted between January and July 2024. A total of 61 pediatric patients aged 0-16 years and admitted to Kafkas University Health Research and Application Hospital were enrolled. Nasopharyngeal swab samples were collected and analyzed using MT-PCR panels. The 7- pathogen panel detected ADV, INF AB, HRV, RSV AB, SARS-CoV-2, and S. pyogenes, while the 24- pathogen panel included an extended range of pathogens, such as HCoV, HPIV, HBoV, S. pneumoniae, and H. influenzae. Comparative analyses focused on positivity rates, co-infections, and pathogen distribution. Results: Pathogens were detected in 96.7 of the samples using the 24-pathogen panel compared to 83.6 with the 7-pathogen panel. Viral pathogens dominated the infections, with RSV AB 10.3 being the most frequently detected pathogen in 24- pathogen panel and INF-B 27.1 in 7-pathogen panel. The 24-pathogen panel identified more bacterial pathogens, notably S. pneumoniae 22.4. Co-infections were significantly higher with the 24-pathogen panel 62.3 compared to the 7-pathogen panel 18.3. Conclusion: The 24-pathogen MT-PCR panel demonstrated superior diagnostic capabilities, highlighting the importance of comprehensive pathogen detection for accurate diagnosis and effective treatment of URTIs. This study underlines the necessity of using advanced molecular diagnostic tools to improve clinical outcomes and support public health strategies in managing respiratory tract infections among pediatric populations.

 Objective: Respiratory tract infections RTIs are among the leading causes of morbidity and mortality in children worldwide. This study aimed to compare the diagnostic performance of two multiplex PCR MT-PCR panels, 7-pathogen and 24- pathogen, for detecting viral and bacterial pathogens in pediatric patients having upper respiratory tract infection URTI symptoms. Methods: The study was conducted between January and July 2024. A total of 61 pediatric patients aged 0-16 years and admitted to Kafkas University Health Research and Application Hospital were enrolled. Nasopharyngeal swab samples were collected and analyzed using MT-PCR panels. The 7- pathogen panel detected ADV, INF AB, HRV, RSV AB, SARS-CoV-2, and S. pyogenes, while the 24- pathogen panel included an extended range of pathogens, such as HCoV, HPIV, HBoV, S. pneumoniae, and H. influenzae. Comparative analyses focused on positivity rates, co-infections, and pathogen distribution. Results: Pathogens were detected in 96.7 of the samples using the 24-pathogen panel compared to 83.6 with the 7-pathogen panel. Viral pathogens dominated the infections, with RSV AB 10.3 being the most frequently detected pathogen in 24- pathogen panel and INF-B 27.1 in 7-pathogen panel. The 24-pathogen panel identified more bacterial pathogens, notably S. pneumoniae 22.4. Co-infections were significantly higher with the 24-pathogen panel 62.3 compared to the 7-pathogen panel 18.3. Conclusion: The 24-pathogen MT-PCR panel demonstrated superior diagnostic capabilities, highlighting the importance of comprehensive pathogen detection for accurate diagnosis and effective treatment of URTIs. This study underlines the necessity of using advanced molecular diagnostic tools to improve clinical outcomes and support public health strategies in managing respiratory tract infections among pediatric populations.

 Objective: Respiratory tract infections RTIs are among the leading causes of morbidity and mortality in children worldwide. This study aimed to compare the diagnostic performance of two multiplex PCR MT-PCR panels, 7-pathogen and 24- pathogen, for detecting viral and bacterial pathogens in pediatric patients having upper respiratory tract infection URTI symptoms. Methods: The study was conducted between January and July 2024. A total of 61 pediatric patients aged 0-16 years and admitted to Kafkas University Health Research and Application Hospital were enrolled. Nasopharyngeal swab samples were collected and analyzed using MT-PCR panels. The 7- pathogen panel detected ADV, INF AB, HRV, RSV AB, SARS-CoV-2, and S. pyogenes, while the 24- pathogen panel included an extended range of pathogens, such as HCoV, HPIV, HBoV, S. pneumoniae, and H. influenzae. Comparative analyses focused on positivity rates, co-infections, and pathogen distribution. Results: Pathogens were detected in 96.7 of the samples using the 24-pathogen panel compared to 83.6 with the 7-pathogen panel. Viral pathogens dominated the infections, with RSV AB 10.3 being the most frequently detected pathogen in 24- pathogen panel and INF-B 27.1 in 7-pathogen panel. The 24-pathogen panel identified more bacterial pathogens, notably S. pneumoniae 22.4. Co-infections were significantly higher with the 24-pathogen panel 62.3 compared to the 7-pathogen panel 18.3. Conclusion: The 24-pathogen MT-PCR panel demonstrated superior diagnostic capabilities, highlighting the importance of comprehensive pathogen detection for accurate diagnosis and effective treatment of URTIs. This study underlines the necessity of using advanced molecular diagnostic tools to improve clinical outcomes and support public health strategies in managing respiratory tract infections among pediatric populations.

 Objective: Respiratory tract infections RTIs are among the leading causes of morbidity and mortality in children worldwide. This study aimed to compare the diagnostic performance of two multiplex PCR MT-PCR panels, 7-pathogen and 24- pathogen, for detecting viral and bacterial pathogens in pediatric patients having upper respiratory tract infection URTI symptoms. Methods: The study was conducted between January and July 2024. A total of 61 pediatric patients aged 0-16 years and admitted to Kafkas University Health Research and Application Hospital were enrolled. Nasopharyngeal swab samples were collected and analyzed using MT-PCR panels. The 7- pathogen panel detected ADV, INF AB, HRV, RSV AB, SARS-CoV-2, and S. pyogenes, while the 24- pathogen panel included an extended range of pathogens, such as HCoV, HPIV, HBoV, S. pneumoniae, and H. influenzae. Comparative analyses focused on positivity rates, co-infections, and pathogen distribution. Results: Pathogens were detected in 96.7 of the samples using the 24-pathogen panel compared to 83.6 with the 7-pathogen panel. Viral pathogens dominated the infections, with RSV AB 10.3 being the most frequently detected pathogen in 24- pathogen panel and INF-B 27.1 in 7-pathogen panel. The 24-pathogen panel identified more bacterial pathogens, notably S. pneumoniae 22.4. Co-infections were significantly higher with the 24-pathogen panel 62.3 compared to the 7-pathogen panel 18.3. Conclusion: The 24-pathogen MT-PCR panel demonstrated superior diagnostic capabilities, highlighting the importance of comprehensive pathogen detection for accurate diagnosis and effective treatment of URTIs. This study underlines the necessity of using advanced molecular diagnostic tools to improve clinical outcomes and support public health strategies in managing respiratory tract infections among pediatric populations.

Makale detayları

The Comparison of Two Different Multiplex Respiratory PCR Panels and The Evaluation of the Viral and Bacterial Agents

Yazarlar:

Nurullah Çiftçi | Gizem Tükenmez | Murat Karameşe

Yayıncı:

Dicle University

Ana Başlıklar:

Tıp Eğitimi | Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi:

Dicle Tıp Dergisi Vol.52 Issue 2, pp.273-281

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001348134

pISSN:

1300-2945

Respiratory Tract Infections

Multiplex PCR

Viral Pathogens

SARS-CoV-2

Bacterial Co-infections

Pediatrics

URTI

Sayı

Makale detayları

Yazarlar: Nurullah Çiftçi | Gizem Tükenmez | Murat Karameşe

Yayıncı: Dicle University

Ana Başlıklar: Tıp Eğitimi | Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi: Dicle Tıp Dergisi Vol.52 Issue 2, pp.273-281

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001348134

pISSN: 1300-2945

Sayı

Yazarlar:

Nurullah Çiftçi | Gizem Tükenmez | Murat Karameşe

Yayıncı:

Dicle University

Ana Başlıklar:

Tıp Eğitimi | Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi:

Dicle Tıp Dergisi Vol.52 Issue 2, pp.273-281

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001348134

pISSN:

1300-2945