Cambial Isoperoxidases Related to Graft Compatibility in Pear-Quince Graft Combinations

Hatice GÜLEN*; Menşure ÇELİK; Mehmet POLAT; Atilla ERİŞ

Makale detayları

Cambial Isoperoxidases Related to Graft Compatibility in Pear-Quince Graft Combinations

Yazarlar:

Hatice GÜLEN* | Menşure ÇELİK | Mehmet POLAT | Atilla ERİŞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2005 Vol.29 Issue 1, pp.83-89

Belge Tipi:

Makale

Yayın Tarihi:

12/2004

Belge Dili:

Türkçe

Document ID (Org No.):

2000809102

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Anahtar kelimeler:

Özet:

 

This study was initiated to survey the graft compatibility characteristics of some selected quince clones known as "S.Ö." with regard to isoperoxidase markers. Compatible (Beurre Hardy = BH) and incompatible (Bartlett = BT) pear cultivars were grafted on quince A (QA) and 13 S.Ö. quince clones by T budding. Bark tissues were collected from the union (4, 8 and 12 weeks after grafting) and from unbudded rootstocks in addition to current-year shoots of 2 pear scions to investigate 2 anodal isoperoxidase bands. Isoperoxidase profiles of the samples were visualized by native polyacrylamide gel electrophoresis and no marked difference was detected among the isoperoxidase profiles of the samples collected 4, 8 and 12 weeks after budding. Many isozyme bands were observed in common in the 2 scions. However, one anodal peroxidase (A, Rf = 0.88) was detected in BH but not in BT samples. This isoperoxidase was also detected in QA and 5 of the S.Ö. quince clones. Isoperoxidase B (Rf = 0.68) was detected in BH but not in BT or any of the rootstocks. Considering the graft union samples, all the combinations with BH contained bands A and B whereas incompatible graft union tissues (BT/QA) lacked both. Graft union samples involving BT and 5 S.Ö. quince clones (35-160, 54-298, 40-214, 58-316 and 58-315) had both isoperoxidases. The data indicated that these 5 S.Ö. quince clones might form compatible graft unions with BT.

 

This study was initiated to survey the graft compatibility characteristics of some selected quince clones known as "S.Ö." with regard to isoperoxidase markers. Compatible (Beurre Hardy = BH) and incompatible (Bartlett = BT) pear cultivars were grafted on quince A (QA) and 13 S.Ö. quince clones by T budding. Bark tissues were collected from the union (4, 8 and 12 weeks after grafting) and from unbudded rootstocks in addition to current-year shoots of 2 pear scions to investigate 2 anodal isoperoxidase bands. Isoperoxidase profiles of the samples were visualized by native polyacrylamide gel electrophoresis and no marked difference was detected among the isoperoxidase profiles of the samples collected 4, 8 and 12 weeks after budding. Many isozyme bands were observed in common in the 2 scions. However, one anodal peroxidase (A, Rf = 0.88) was detected in BH but not in BT samples. This isoperoxidase was also detected in QA and 5 of the S.Ö. quince clones. Isoperoxidase B (Rf = 0.68) was detected in BH but not in BT or any of the rootstocks. Considering the graft union samples, all the combinations with BH contained bands A and B whereas incompatible graft union tissues (BT/QA) lacked both. Graft union samples involving BT and 5 S.Ö. quince clones (35-160, 54-298, 40-214, 58-316 and 58-315) had both isoperoxidases. The data indicated that these 5 S.Ö. quince clones might form compatible graft unions with BT.

Makale detayları

Cambial Isoperoxidases Related to Graft Compatibility in Pear-Quince Graft Combinations

Yazarlar:

Hatice GÜLEN* | Menşure ÇELİK | Mehmet POLAT | Atilla ERİŞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2005 Vol.29 Issue 1, pp.83-89

Belge Tipi:

Makale

Yayın Tarihi:

12/2004

Belge Dili:

Türkçe

Document ID (Org No.):

2000809102

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Quince (Cydonia oblanga)

grafting

incompatibility

peroxidase

pear (Pyrus communis).

Sayı

Makale detayları

Cambial Isoperoxidases Related to Graft Compatibility in Pear-Quince Graft Combinations

Yazarlar: Hatice GÜLEN* | Menşure ÇELİK | Mehmet POLAT | Atilla ERİŞ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Agriculture and Forestry 2005 Vol.29 Issue 1, pp.83-89

Belge Tipi: Makale

Yayın Tarihi: 12/2004

Belge Dili: Türkçe

Document ID (Org No.): 2000809102

Sıklık: İki Aylık

eISSN: 1303-6173

pISSN: 1300-011X

Quince (Cydonia oblanga)

grafting

incompatibility

peroxidase

pear (Pyrus communis).

Sayı

Yazarlar:

Hatice GÜLEN* | Menşure ÇELİK | Mehmet POLAT | Atilla ERİŞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2005 Vol.29 Issue 1, pp.83-89

Belge Tipi:

Makale

Yayın Tarihi:

12/2004

Belge Dili:

Türkçe

Document ID (Org No.):

2000809102

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X