Genoprotective potential of Brassica juncea (L.) Czern. against mercury-induced genotoxicity in Allium cepa L.

Sonia SHARMA; Avinash NAGPAL; Adarsh Pal VIG

Makale detayları

Genoprotective potential of Brassica juncea (L.) Czern. against mercury-induced genotoxicity in Allium cepa L.

Yazarlar:

Sonia SHARMA | Avinash NAGPAL | Adarsh Pal VIG

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2012 Vol.36 Issue 6, pp.622-629

Belge Tipi:

Makale

Yayın Tarihi:

5/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000812538

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

Brassica juncea (L.) Czern. is an important species of the family Brassicaceae with immense medicinal value. The main goal of this study was to investigate the antigenotoxic effects of different concentrations (0.1%-1.0%) of chloroform extract of B. juncea seeds on mercury-induced (0.75 ppm) genotoxic effects in root cells of Allium cepa. Three different modes of treatment were performed: pretreatment, posttreatment, and simultaneous treatment. For pretreatment, freshly emerged root tips were first treated with different concentrations of seed extract for 3 h and then treated with Hg for 3 h. For posttreatment, the roots were first treated with Hg and then with extract concentrations. For simultaneous treatment, different concentrations of extract were added along with the Hg. The study revealed that chloroform extract was neither toxic nor in possession of genotoxic activity. Treatment with Hg caused a decrease in mitotic index and induction of chromosomal aberrations as compared to the negative control. This effect was reversed by treatment with different concentrations of chloroform extract of seeds of B. juncea. Maximum percentage inhibition (95.3%) of genotoxic effects was observed with 1% chloroform extract (post- and simultaneous treatment). This study clearly indicates that the chloroform extract of B. juncea seeds has antigenotoxic potential against mercury-induced genotoxicity in a dose-dependent manner by evaluating A. cepa root chromosomal aberration assay.

 

Brassica juncea (L.) Czern. is an important species of the family Brassicaceae with immense medicinal value. The main goal of this study was to investigate the antigenotoxic effects of different concentrations (0.1%-1.0%) of chloroform extract of B. juncea seeds on mercury-induced (0.75 ppm) genotoxic effects in root cells of Allium cepa. Three different modes of treatment were performed: pretreatment, posttreatment, and simultaneous treatment. For pretreatment, freshly emerged root tips were first treated with different concentrations of seed extract for 3 h and then treated with Hg for 3 h. For posttreatment, the roots were first treated with Hg and then with extract concentrations. For simultaneous treatment, different concentrations of extract were added along with the Hg. The study revealed that chloroform extract was neither toxic nor in possession of genotoxic activity. Treatment with Hg caused a decrease in mitotic index and induction of chromosomal aberrations as compared to the negative control. This effect was reversed by treatment with different concentrations of chloroform extract of seeds of B. juncea. Maximum percentage inhibition (95.3%) of genotoxic effects was observed with 1% chloroform extract (post- and simultaneous treatment). This study clearly indicates that the chloroform extract of B. juncea seeds has antigenotoxic potential against mercury-induced genotoxicity in a dose-dependent manner by evaluating A. cepa root chromosomal aberration assay.

Makale detayları

Genoprotective potential of Brassica juncea (L.) Czern. against mercury-induced genotoxicity in Allium cepa L.

Yazarlar:

Sonia SHARMA | Avinash NAGPAL | Adarsh Pal VIG

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2012 Vol.36 Issue 6, pp.622-629

Belge Tipi:

Makale

Yayın Tarihi:

5/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000812538

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Key words: Antigenotoxicity

chloroform extract

Allium cepa root chromosomal aberration assay

Sayı

Makale detayları

Genoprotective potential of Brassica juncea (L.) Czern. against mercury-induced genotoxicity in Allium cepa L.

Yazarlar: Sonia SHARMA | Avinash NAGPAL | Adarsh Pal VIG

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2012 Vol.36 Issue 6, pp.622-629

Belge Tipi: Makale

Yayın Tarihi: 5/2012

Belge Dili: Türkçe

Document ID (Org No.): 2000812538

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Key words: Antigenotoxicity

chloroform extract

Allium cepa root chromosomal aberration assay

Sayı

Yazarlar:

Sonia SHARMA | Avinash NAGPAL | Adarsh Pal VIG

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2012 Vol.36 Issue 6, pp.622-629

Belge Tipi:

Makale

Yayın Tarihi:

5/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000812538

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152