WHAT DO PEOPLE PREFER TO SUPPORT DIABETES TREATMENT IN TURKIYE? A STUDY ON OLIVE LEAF AND DIABETES

Methiye Mancak; Ufuk Koca Çalışkan

Makale detayları

WHAT DO PEOPLE PREFER TO SUPPORT DIABETES TREATMENT IN TURKIYE? A STUDY ON OLIVE LEAF AND DIABETES

Yazarlar:

Methiye Mancak | Ufuk Koca Çalışkan

Yayıncı:

Ankara University

Ana Başlıklar:

Farmakognozi

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 2, pp.436-455

Belge Tipi:

Makale

Yayın Tarihi:

5/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001311399

pISSN:

1015-3918

Anahtar kelimeler:

Özet:

 Objective: A questionnaire study was conducted to evaluate the applications of plants and herbal products consumed by diabetic patients for the treatment of chronic health conditions. Evaluation of the questionnaire confirmed that olive leaf is one of the most used herbs in the treatment of diabetes, in line with its traditional use in the literature. In vitro biological activity studies were performed to determine whether different olive leaf samples have antidiabetic effects. Additionally, the major component oleuropein was quantitively determined in the samples. Material and Method: The established survey was firstly approved by the ethics committee at Gazi University then the survey was conducted at the University Hospital, Department of Endocrinology between January 2021, and July 2021. Based on the result of the survey, pharmacognostic analyses, chromatographic analyses, and inhibition on diabetes-related enzymes α-amylase, α-glucosidase, and aldose reductase were performed on the samples of olive leaves collected from nature, obtained from herbalists, markets and pharmacies. Result and Discussion: Evaluation of the survey revealed that the patients mostly used cinnamon 29.3 and olive leaves 21.7 for the treatment of diabetes, and these plants were generally obtained from herbalists 51.7. The study findings showed that aqueous and ethanolic extracts prepared from olive leaf samples contained 190.3-374.3 mgg oleuropein. The amount of oleuropein in the ready-made olive leaf extract from herbalists was found to be much lower 50.9 mgg than the other olive leaf extracts. When the enzyme inhibition activity assays were evaluated, it was determined that all olive leaf samples had inhibitory effects on α-amylase, α-glucosidase, and aldose reductase enzymes. All olive leaf samples, including teas prepared by the public at home with water, were found to have capacity to decrease the blood level in other words antidiabetic activities in vitro. The oleuropein contents detected in this study once again revealed the importance of meticulous examination in herbal products.

 Objective: A questionnaire study was conducted to evaluate the applications of plants and herbal products consumed by diabetic patients for the treatment of chronic health conditions. Evaluation of the questionnaire confirmed that olive leaf is one of the most used herbs in the treatment of diabetes, in line with its traditional use in the literature. In vitro biological activity studies were performed to determine whether different olive leaf samples have antidiabetic effects. Additionally, the major component oleuropein was quantitively determined in the samples. Material and Method: The established survey was firstly approved by the ethics committee at Gazi University then the survey was conducted at the University Hospital, Department of Endocrinology between January 2021, and July 2021. Based on the result of the survey, pharmacognostic analyses, chromatographic analyses, and inhibition on diabetes-related enzymes α-amylase, α-glucosidase, and aldose reductase were performed on the samples of olive leaves collected from nature, obtained from herbalists, markets and pharmacies. Result and Discussion: Evaluation of the survey revealed that the patients mostly used cinnamon 29.3 and olive leaves 21.7 for the treatment of diabetes, and these plants were generally obtained from herbalists 51.7. The study findings showed that aqueous and ethanolic extracts prepared from olive leaf samples contained 190.3-374.3 mgg oleuropein. The amount of oleuropein in the ready-made olive leaf extract from herbalists was found to be much lower 50.9 mgg than the other olive leaf extracts. When the enzyme inhibition activity assays were evaluated, it was determined that all olive leaf samples had inhibitory effects on α-amylase, α-glucosidase, and aldose reductase enzymes. All olive leaf samples, including teas prepared by the public at home with water, were found to have capacity to decrease the blood level in other words antidiabetic activities in vitro. The oleuropein contents detected in this study once again revealed the importance of meticulous examination in herbal products.

 Objective: A questionnaire study was conducted to evaluate the applications of plants and herbal products consumed by diabetic patients for the treatment of chronic health conditions. Evaluation of the questionnaire confirmed that olive leaf is one of the most used herbs in the treatment of diabetes, in line with its traditional use in the literature. In vitro biological activity studies were performed to determine whether different olive leaf samples have antidiabetic effects. Additionally, the major component oleuropein was quantitively determined in the samples. Material and Method: The established survey was firstly approved by the ethics committee at Gazi University then the survey was conducted at the University Hospital, Department of Endocrinology between January 2021, and July 2021. Based on the result of the survey, pharmacognostic analyses, chromatographic analyses, and inhibition on diabetes-related enzymes α-amylase, α-glucosidase, and aldose reductase were performed on the samples of olive leaves collected from nature, obtained from herbalists, markets and pharmacies. Result and Discussion: Evaluation of the survey revealed that the patients mostly used cinnamon 29.3 and olive leaves 21.7 for the treatment of diabetes, and these plants were generally obtained from herbalists 51.7. The study findings showed that aqueous and ethanolic extracts prepared from olive leaf samples contained 190.3-374.3 mgg oleuropein. The amount of oleuropein in the ready-made olive leaf extract from herbalists was found to be much lower 50.9 mgg than the other olive leaf extracts. When the enzyme inhibition activity assays were evaluated, it was determined that all olive leaf samples had inhibitory effects on α-amylase, α-glucosidase, and aldose reductase enzymes. All olive leaf samples, including teas prepared by the public at home with water, were found to have capacity to decrease the blood level in other words antidiabetic activities in vitro. The oleuropein contents detected in this study once again revealed the importance of meticulous examination in herbal products.

 Amaç: Diyabet hastalarının tükettikleri bitki ve bitkisel ürünlerin kronik sağlık durumlarının tedavisine yönelik uygulamalarının değerlendirilmesi amacıyla bir anket çalışması yapılmıştır. Anket sonuçları, literatürdeki geleneksel kullanımına paralel olarak zeytin yaprağının diyabet tedavisinde en çok kullanılan bitkilerden biri olduğunu doğrulamıştır. Farklı zeytin yaprağı örneklerinin antidiyabetik etkisinin olup olmadığının belirlenmesi amacıyla in vitro biyolojik aktivite çalışmaları yapılmış ve ana bileşen oleuropein kantitatif olarak belirlenmiştir. Gereç ve Yöntem: Anket için öncelikle Gazi Üniversitesi Etik kurulu tarafından onay alınmış, Ocak 2021-Temmuz 2021 tarihleri arasında Gazi Üniversitesi Endokrinoloji Bölümünde uygulanmıştır. Çalışmaya 18 yaş üstü Tip 1 ve Tip 2 diyabetli 200 hasta dahil edilmiştir. Anket sonuçlarından yola çıkarak, doğadan toplanan, aktarlardan, marketlerden ve eczanelerden temin edilen zeytin yaprağı örnekleri üzerinde farmakognozik analizler makroskopik ve mikroskobik analiz, toplam kül, kuruma kaybı, kromatografik analizler ince tabaka kromatografisi ve yüksek basınçlı sıvı kromatografisi analizleri ve diyabetle ilişkili enzimlerin α-amilaz, α-glukosidaz ve aldoz redüktaz inhibisyonu çalışmaları yapılmıştır. Sonuç ve Tartışma: Anketin değerlendirilmesinde hastaların diyabet tedavisi için en çok tarçın 29.3 ve zeytin yaprağını 21.7 kullandığı ve bu bitkilerin genellikle aktarlardan 51.7 temin edildiği görülmüştür. Çalışma bulguları, zeytin yaprağı örneklerinden hazırlanan sulu ve etanol ekstraktlarının 190.3-374.3 mgg oleuropein içerdiğini göstermiştir. Aktarlardan alınan hazır zeytin yaprağı ekstraktındaki oleuropein miktarı diğer zeytin yaprağı ekstraktlarına göre çok daha düşük 50.9 mgg bulunmuştur. Enzim inhibisyon aktivite testleri değerlendirildiğinde, tüm zeytin yaprağı örneklerinin α-amilaz, α-glukosidaz ve aldoz redüktaz enzimleri üzerinde inhibitör etkiye sahip olduğu belirlenmiştir. Halk tarafından evde su ile hazırlanan çaylar da dahil olmak üzere tüm zeytin yaprağı örneklerinin in vitro kan seviyesini düşürme yani antidiyabetik aktiviteye sahip olduğu tespit edilmiştir. Bu çalışmada tespit edilen oleuropein içerikleri bitkisel ürünlerde titiz incelemenin önemini bir kez daha ortaya koymuştur.

Makale detayları

WHAT DO PEOPLE PREFER TO SUPPORT DIABETES TREATMENT IN TURKIYE? A STUDY ON OLIVE LEAF AND DIABETES

Yazarlar:

Methiye Mancak | Ufuk Koca Çalışkan

Yayıncı:

Ankara University

Ana Başlıklar:

Farmakognozi

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 2, pp.436-455

Belge Tipi:

Makale

Yayın Tarihi:

5/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001311399

pISSN:

1015-3918

Aldose reductase

α-amylase

α-glucosidase

high pressure liquid chromatography

Olea europeaAldoz redüktaz

α-amilaz

α-glukozidaz

Olea europea

yüksek performanslı sıvı kromatografisi

Sayı

Makale detayları

Yazarlar: Methiye Mancak | Ufuk Koca Çalışkan

Yayıncı: Ankara University

Ana Başlıklar: Farmakognozi

Dergi: Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 2, pp.436-455

Belge Tipi: Makale

Yayın Tarihi: 5/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001311399

pISSN: 1015-3918

Sayı

Yazarlar:

Methiye Mancak | Ufuk Koca Çalışkan

Yayıncı:

Ankara University

Ana Başlıklar:

Farmakognozi

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 2, pp.436-455

Belge Tipi:

Makale

Yayın Tarihi:

5/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001311399

pISSN:

1015-3918