The Effects of Gonadotrophins on In Vitro Maturation of Cat Oocytes

Mithat EVECEN; Alper BARAN

Makale detayları

The Effects of Gonadotrophins on In Vitro Maturation of Cat Oocytes

Yazarlar:

Mithat EVECEN | Alper BARAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2002 Vol.26 Issue 6, pp.1315-1320

Belge Tipi:

Makale

Yayın Tarihi:

5/2002

Belge Dili:

Türkçe

Document ID (Org No.):

2000770412

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

The aim of this study was to determine the effects of FSH and LH hormones on in vitro development of cat oocytes. The ovaries from spayed cats were brought to the laboratory in +4 °C PBS solution two hours after being collected. The oocytes were recovered by slicing of ovaries and were separated into two groups: Group 1 (Control); TCM 199 + 5% FCS + 2.2 g/l NaHCO3 + 0.23 mM Na Pyruvate; and Group 2 (Treatment); TCM 199 + 5% FCS + 2.2 g/l NaHCO3 + 0.23mM Na Pyruvate + FSH (Sigma F-2293) (1 µg/ml) + LH (Sigma L-5269) (1 µg/ml). Oocytes were matured for 48 hours at 38.5 °C and 5% CO2 in a humidified atmosphere. At the end of this period oocytes were fixed, stained and evaluated for maturation criteria by phase-contrast microscope at x400 magnification. Data were analyzed statistically by Student's t - test.     A total of 428 oocytes (Control: 203; Treatment: 225) were used. Eleven of the control group oocytes were at M I (5.4%), 2 (0.98%) were at anaphase I and 2 (0.98%) were at M II stage. These values for the treatment group were 25 (11.11%), 0 (0.00%) and 6 (2.66%), respectively. There was no statistical difference between the control and hormone added groups (p>0.05). For the oocytes which continued to undergo meiosis, the difference between the groups (Control: 22.66%; Treatment: 31.55%) was statistically significant (p.

 

The aim of this study was to determine the effects of FSH and LH hormones on in vitro development of cat oocytes. The ovaries from spayed cats were brought to the laboratory in +4 °C PBS solution two hours after being collected. The oocytes were recovered by slicing of ovaries and were separated into two groups: Group 1 (Control); TCM 199 + 5% FCS + 2.2 g/l NaHCO3 + 0.23 mM Na Pyruvate; and Group 2 (Treatment); TCM 199 + 5% FCS + 2.2 g/l NaHCO3 + 0.23mM Na Pyruvate + FSH (Sigma F-2293) (1 µg/ml) + LH (Sigma L-5269) (1 µg/ml). Oocytes were matured for 48 hours at 38.5 °C and 5% CO2 in a humidified atmosphere. At the end of this period oocytes were fixed, stained and evaluated for maturation criteria by phase-contrast microscope at x400 magnification. Data were analyzed statistically by Student's t - test.     A total of 428 oocytes (Control: 203; Treatment: 225) were used. Eleven of the control group oocytes were at M I (5.4%), 2 (0.98%) were at anaphase I and 2 (0.98%) were at M II stage. These values for the treatment group were 25 (11.11%), 0 (0.00%) and 6 (2.66%), respectively. There was no statistical difference between the control and hormone added groups (p>0.05). For the oocytes which continued to undergo meiosis, the difference between the groups (Control: 22.66%; Treatment: 31.55%) was statistically significant (p.

Makale detayları

The Effects of Gonadotrophins on In Vitro Maturation of Cat Oocytes

Yazarlar: Mithat EVECEN | Alper BARAN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2002 Vol.26 Issue 6, pp.1315-1320

Belge Tipi: Makale

Yayın Tarihi: 5/2002

Belge Dili: Türkçe

Document ID (Org No.): 2000770412

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Cat

Oocyte

In Vitro Maturation

Gonadotrophins

Sayı

Yazarlar:

Mithat EVECEN | Alper BARAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2002 Vol.26 Issue 6, pp.1315-1320

Belge Tipi:

Makale

Yayın Tarihi:

5/2002

Belge Dili:

Türkçe

Document ID (Org No.):

2000770412

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128