Evaluation of canine anti-Leishmania IgG subclasses and their relation with skin signs in naturally infected dogs in the northwest of Iran

Ali Shabestari ASL; Majid SHARIFI; Mehdi MOHEBALI; Zabihollah ZAREI; Zohreh BABALOO; Behnaz AKHOUNDI

Makale detayları

Evaluation of canine anti-Leishmania IgG subclasses and their relation with skin signs in naturally infected dogs in the northwest of Iran

Yazarlar:

Ali Shabestari ASL | Majid SHARIFI | Mehdi MOHEBALI | Zabihollah ZAREI | Zohreh BABALOO | Behnaz AKHOUNDI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2013 Vol.37 Issue 5, pp.512-515

Belge Tipi:

Makale

Yayın Tarihi:

4/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000766270

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

Canine leishmaniasis (CanL) is a severe, intracellular parasitic zoonosis and is an endemic complaint in Iran. Dogs are considered the reservoir host for Leishmania infantum in the Old World. The aims of this study were to evaluate the relation between anti-Leishmania antibody titers (IgG) and skin symptoms of CanL in naturally affected dogs and to assess the correlation of IgG1 and IgG2 subclasses of titers with these symptoms. Anti-Leishmania antibody titers were evaluated in 100 dogs' blood samples with the direct agglutination test (DAT) and enzyme-linked immunosorbent assay (ELISA) methods. Based on DAT results, 45 samples were positive. Twenty-five of the positive dogs had cutaneous signs of leishmaniasis and 20 of them were asymptomatic (DAT of 1:320 and above). In the dogs, high levels of total IgG were found in symptomatic dogs. In ELISA, we found an apparent correlation between IgG1 and symptomatic dogs and between IgG2 and asymptomatic dogs. It seems that measurements of IgG subclasses can be used as gold-standard markers for evaluation of susceptibility or resistance to this lethal chronic infectious disease in dogs.

 

Canine leishmaniasis (CanL) is a severe, intracellular parasitic zoonosis and is an endemic complaint in Iran. Dogs are considered the reservoir host for Leishmania infantum in the Old World. The aims of this study were to evaluate the relation between anti-Leishmania antibody titers (IgG) and skin symptoms of CanL in naturally affected dogs and to assess the correlation of IgG1 and IgG2 subclasses of titers with these symptoms. Anti-Leishmania antibody titers were evaluated in 100 dogs' blood samples with the direct agglutination test (DAT) and enzyme-linked immunosorbent assay (ELISA) methods. Based on DAT results, 45 samples were positive. Twenty-five of the positive dogs had cutaneous signs of leishmaniasis and 20 of them were asymptomatic (DAT of 1:320 and above). In the dogs, high levels of total IgG were found in symptomatic dogs. In ELISA, we found an apparent correlation between IgG1 and symptomatic dogs and between IgG2 and asymptomatic dogs. It seems that measurements of IgG subclasses can be used as gold-standard markers for evaluation of susceptibility or resistance to this lethal chronic infectious disease in dogs.

Makale detayları

Evaluation of canine anti-Leishmania IgG subclasses and their relation with skin signs in naturally infected dogs in the northwest of Iran

Yazarlar:

Ali Shabestari ASL | Majid SHARIFI | Mehdi MOHEBALI | Zabihollah ZAREI | Zohreh BABALOO | Behnaz AKHOUNDI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2013 Vol.37 Issue 5, pp.512-515

Belge Tipi:

Makale

Yayın Tarihi:

4/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000766270

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Key words: Canine leishmaniasis

immunoglobulin G

direct agglutination test

ELISA

Sayı

Makale detayları

Evaluation of canine anti-Leishmania IgG subclasses and their relation with skin signs in naturally infected dogs in the northwest of Iran

Yazarlar: Ali Shabestari ASL | Majid SHARIFI | Mehdi MOHEBALI | Zabihollah ZAREI | Zohreh BABALOO | Behnaz AKHOUNDI

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2013 Vol.37 Issue 5, pp.512-515

Belge Tipi: Makale

Yayın Tarihi: 4/2013

Belge Dili: Türkçe

Document ID (Org No.): 2000766270

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Key words: Canine leishmaniasis

immunoglobulin G

direct agglutination test

ELISA

Sayı

Yazarlar:

Ali Shabestari ASL | Majid SHARIFI | Mehdi MOHEBALI | Zabihollah ZAREI | Zohreh BABALOO | Behnaz AKHOUNDI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2013 Vol.37 Issue 5, pp.512-515

Belge Tipi:

Makale

Yayın Tarihi:

4/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000766270

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128