The Effect of Apocynin on Ischemia Modified Albumin (IMA) Levels in the Ataxia Model Induced with 3-Acetylpyridine in Rats

Ömercan Alat; Elif Dalkılınç

Makale detayları

The Effect of Apocynin on Ischemia Modified Albumin (IMA) Levels in the Ataxia Model Induced with 3-Acetylpyridine in Rats

Yazarlar:

Ömercan Alat | Elif Dalkılınç

Yayıncı:

Ataturk University

Ana Başlıklar:

Biyokimya ve Hücre Biyolojisi (Diğer) | Sağlık Kurumları Yönetimi

Dergi:

Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi Vol.5 Issue 2, pp.67-72

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001358086

pISSN:

2791-8645

Anahtar kelimeler:

Özet:

 Cerebellar ataxia is a neurodegenerative disease that causes coordination disorder. In such neurodegenerative diseases, compounds such as apocynin APO have a therapeutic role. The aim of this study is to examine the effect of APO treatment on ischemia modified albumin IMA level in the cerebellar ataxia model induced by 3-acetylpyridine 3-AP. In the study, 56-week-old male Sprague-Dawley rats weighing 200-250 grams were used. Rats were divided into four groups: Control group K, 3- 3-AP group, 3-AP  APO, and APO group. A cerebellar ataxia model was induced in rats using 3-AP administration, and APO was administered as a treatment. At the end of the experiment, decapitation was performed under anesthesia, and IMA levels were measured in the brain tissues obtained. In rats administered with 3-AP, there was a statistically significant increase in IMA levels compared to all groups, whereas rats treated with APO showed a statistically significant decrease in IMA levels p0.001. Although many studies in recent years have investigated the usability of IMA levels as biomarkers, literature searches did not reveal any studies examining the therapeutic potential of APO in neurodegenerative diseases like cerebellar ataxia induced by 3-AP, in terms of brain IMA levels. Therefore, this study is presented as a pioneering contribution to the literature. The findings obtained shed light on future research.

 Cerebellar ataxia is a neurodegenerative disease that causes coordination disorder. In such neurodegenerative diseases, compounds such as apocynin APO have a therapeutic role. The aim of this study is to examine the effect of APO treatment on ischemia modified albumin IMA level in the cerebellar ataxia model induced by 3-acetylpyridine 3-AP. In the study, 56-week-old male Sprague-Dawley rats weighing 200-250 grams were used. Rats were divided into four groups: Control group K, 3- 3-AP group, 3-AP  APO, and APO group. A cerebellar ataxia model was induced in rats using 3-AP administration, and APO was administered as a treatment. At the end of the experiment, decapitation was performed under anesthesia, and IMA levels were measured in the brain tissues obtained. In rats administered with 3-AP, there was a statistically significant increase in IMA levels compared to all groups, whereas rats treated with APO showed a statistically significant decrease in IMA levels p0.001. Although many studies in recent years have investigated the usability of IMA levels as biomarkers, literature searches did not reveal any studies examining the therapeutic potential of APO in neurodegenerative diseases like cerebellar ataxia induced by 3-AP, in terms of brain IMA levels. Therefore, this study is presented as a pioneering contribution to the literature. The findings obtained shed light on future research.

 Cerebellar ataxia is a neurodegenerative disease that causes coordination disorder. In such neurodegenerative diseases, compounds such as apocynin APO have a therapeutic role. The aim of this study is to examine the effect of APO treatment on ischemia modified albumin IMA level in the cerebellar ataxia model induced by 3-acetylpyridine 3-AP. In the study, 56-week-old male Sprague-Dawley rats weighing 200-250 grams were used. Rats were divided into four groups: Control group K, 3- 3-AP group, 3-AP  APO, and APO group. A cerebellar ataxia model was induced in rats using 3-AP administration, and APO was administered as a treatment. At the end of the experiment, decapitation was performed under anesthesia, and IMA levels were measured in the brain tissues obtained. In rats administered with 3-AP, there was a statistically significant increase in IMA levels compared to all groups, whereas rats treated with APO showed a statistically significant decrease in IMA levels p0.001. Although many studies in recent years have investigated the usability of IMA levels as biomarkers, literature searches did not reveal any studies examining the therapeutic potential of APO in neurodegenerative diseases like cerebellar ataxia induced by 3-AP, in terms of brain IMA levels. Therefore, this study is presented as a pioneering contribution to the literature. The findings obtained shed light on future research.

 Serebellar ataksi koordinasyon bozukluğuna sebep olan nörodejenereatif bir hastalıktır. Bunun gibi nörodejeneratif hastalıklarda apocynin APO gibi bileşiklerin tedavi edici rolü bulunmaktadır. Yapılan çalışmanın amacı, 3-asetilpyridin 3-AP ile oluşturulan serebellar ataksi modelinde APO tedavisinin iskemi modifiye albümin İMA seviyesi üzerindeki etkisini incelemektir. Çalışmada, 200250 gr ağırlığında Sprague-Dawley cinsi, 5-6 haftalık erkek ratlar kullanıldı. Ratlar, Kontrol grubu, 3-AP grubu, 3-AP  APO grubu ve APO grubu olmak üzere dört gruba ayrıldı. 3-AP uygulaması ile ratlarda serebellar ataksi modeli oluşturuldu ve tedavi için APO uygulandı. Deney sonunda anestezi altında dekapitasyon gerçekleştirilerek alınan beyin dokularında İMA seviyeleri ölçüldü. Tüm gruplara göre 3-AP uygulanan ratlarda İMA düzeylerinde istatistiksel olarak anlamlı artış saptanırken, APO uygulanan ratlarda İMA seviyelerinde istatistiksel olarak anlamlı azalış tespit edildi p0.001. Son yıllarda biomarker olarak İMA düzeylerinin kullanılabilirliği yönünde birçok çalışma olsada yapılan literatür taramalarında APOnun 3-AP uygulaması sonucu serebellar ataksi gibi nörodejeneratif hastalıklarda terapötik bir bileşik olarak kullanılabileceğine yönelik beyin İMA düzeylerine bakılan herhangi bir literature rastlanılmadığı için yapılan çalışma literatüre öncü çalışma olarak sunulmuştur. Elde edilen bulgular yapılacak çalışmalar için ışık tutar niteliktedir.

Makale detayları

The Effect of Apocynin on Ischemia Modified Albumin (IMA) Levels in the Ataxia Model Induced with 3-Acetylpyridine in Rats

Yazarlar:

Ömercan Alat | Elif Dalkılınç

Yayıncı:

Ataturk University

Ana Başlıklar:

Biyokimya ve Hücre Biyolojisi (Diğer) | Sağlık Kurumları Yönetimi

Dergi:

Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi Vol.5 Issue 2, pp.67-72

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001358086

pISSN:

2791-8645

Antioxidant

apocynin

ataxia

brain

IMAAntioksidan

aposinin

ataksi

beyin

İMA

Sayı

Makale detayları

Yazarlar: Ömercan Alat | Elif Dalkılınç

Yayıncı: Ataturk University

Ana Başlıklar: Biyokimya ve Hücre Biyolojisi (Diğer) | Sağlık Kurumları Yönetimi

Dergi: Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi Vol.5 Issue 2, pp.67-72

Belge Tipi: Makale

Yayın Tarihi: 9/2025

Belge Dili: Türkçe

Document ID (Org No.): 2001358086

pISSN: 2791-8645

Sayı

Yazarlar:

Ömercan Alat | Elif Dalkılınç

Yayıncı:

Ataturk University

Ana Başlıklar:

Biyokimya ve Hücre Biyolojisi (Diğer) | Sağlık Kurumları Yönetimi

Dergi:

Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi Vol.5 Issue 2, pp.67-72

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001358086

pISSN:

2791-8645