Effect of Kefir Consumption on Lipid Metabolism and Adipokine Hormones in BALB/C Mice Fed A High-Fat Diet

Seren Orhan; Hülya Çiçek; Mustafa Örkmez; Mehmet Akif Bozdayı; Efsane Yavuz; Davut Sinan Kaplan; Ahmet Sarper Bozkurt

Makale detayları

Effect of Kefir Consumption on Lipid Metabolism and Adipokine Hormones in BALB/C Mice Fed A High-Fat Diet

Yazarlar:

Seren Orhan | Hülya Çiçek | Mustafa Örkmez | Mehmet Akif Bozdayı | Efsane Yavuz | Davut Sinan Kaplan | Ahmet Sarper Bozkurt

Yayıncı:

Harran University

Ana Başlıklar:

Tıbbi biyokimya - Proteinler | Peptitler ve Proteomik

Dergi:

Harran Üniversitesi Tıp Fakültesi Dergisi Vol.20 Issue 3, pp.678-683

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001298518

pISSN:

1304-9623

Anahtar kelimeler:

Özet:

 Background: The imbalance in pro-inflammatory and anti-inflammatory hormones secreted by the increase in fat tissue causes chronic inflammation in the fat tissue. It is thought that this chronic inflammation causes metabolic complications result-ing from obesity. Kefir is a type of probiotic that has recently attracted attention in the fight against obesity. This study aimed to examine the effects of kefir consumption on lipid profile and Adiponectin, Leptin, Resistin and IrisinFNDC5 in the high fat diet fed BALBC mouse model. Materials and Methods: BALBC strain male mice were divided into three groups: control group n  10, high fat diet HFD n  10 and HFD  Kefir n  10. Mice were fed specific dietary patterns for eight weeks. The control group was given standard pellet feed. The HFD group was given a high-fat diet containing 52 fat. In addition to the high-fat feed, 15 mlkg kefir was given to the HFDKefir group via oral gavage. Lipid profile was measured on an autoanalyzer using commercial kits. Leptin, Adiponectin, Resistin and IrisinFNDC5 levels were measured by enzyme-linked immunosorbent assay ELISA using commer-cially available kits. Results: As a result of the experiment, there was no difference between the live weight gains of the groups. Epididymal fat weights in the HFD and HFDKefir groups were found to be statistically significantly higher than the control group. There was no significant difference between the epididymal fat weights of the HFD and HFDKefir groups HDL Cholesterol HDL-C, LDL Cholesterol LDL-C values in the HFD and HFDKefir groups were found to be statistically significantly higher than the control group. No statistically significant difference was detected between the HFDKefir group and the HFD group in terms of HDL-C, LDL-C values. There was no difference between groups in triglyceride values. Adiponectin and IrisinFNDC5 values of the HFDKefir group were found to be statistically significantly lower than the other groups. There was no statistically significant difference between the control group and the HFD group in terms of Adiponectin and IrisinFNDC5 values. There was no significant difference between the groups in Leptin and Resistin values. Conclusions: It was thought that kefir may have metabolic effects through adipokines in the high-fat diet nutrition model, and it would be useful to support this with human studies.

 Background: The imbalance in pro-inflammatory and anti-inflammatory hormones secreted by the increase in fat tissue causes chronic inflammation in the fat tissue. It is thought that this chronic inflammation causes metabolic complications result-ing from obesity. Kefir is a type of probiotic that has recently attracted attention in the fight against obesity. This study aimed to examine the effects of kefir consumption on lipid profile and Adiponectin, Leptin, Resistin and IrisinFNDC5 in the high fat diet fed BALBC mouse model. Materials and Methods: BALBC strain male mice were divided into three groups: control group n  10, high fat diet HFD n  10 and HFD  Kefir n  10. Mice were fed specific dietary patterns for eight weeks. The control group was given standard pellet feed. The HFD group was given a high-fat diet containing 52 fat. In addition to the high-fat feed, 15 mlkg kefir was given to the HFDKefir group via oral gavage. Lipid profile was measured on an autoanalyzer using commercial kits. Leptin, Adiponectin, Resistin and IrisinFNDC5 levels were measured by enzyme-linked immunosorbent assay ELISA using commer-cially available kits. Results: As a result of the experiment, there was no difference between the live weight gains of the groups. Epididymal fat weights in the HFD and HFDKefir groups were found to be statistically significantly higher than the control group. There was no significant difference between the epididymal fat weights of the HFD and HFDKefir groups HDL Cholesterol HDL-C, LDL Cholesterol LDL-C values in the HFD and HFDKefir groups were found to be statistically significantly higher than the control group. No statistically significant difference was detected between the HFDKefir group and the HFD group in terms of HDL-C, LDL-C values. There was no difference between groups in triglyceride values. Adiponectin and IrisinFNDC5 values of the HFDKefir group were found to be statistically significantly lower than the other groups. There was no statistically significant difference between the control group and the HFD group in terms of Adiponectin and IrisinFNDC5 values. There was no significant difference between the groups in Leptin and Resistin values. Conclusions: It was thought that kefir may have metabolic effects through adipokines in the high-fat diet nutrition model, and it would be useful to support this with human studies.

 Background: The imbalance in pro-inflammatory and anti-inflammatory hormones secreted by the increase in fat tissue causes chronic inflammation in the fat tissue. It is thought that this chronic inflammation causes metabolic complications result-ing from obesity. Kefir is a type of probiotic that has recently attracted attention in the fight against obesity. This study aimed to examine the effects of kefir consumption on lipid profile and Adiponectin, Leptin, Resistin and IrisinFNDC5 in the high fat diet fed BALBC mouse model. Materials and Methods: BALBC strain male mice were divided into three groups: control group n  10, high fat diet HFD n  10 and HFD  Kefir n  10. Mice were fed specific dietary patterns for eight weeks. The control group was given standard pellet feed. The HFD group was given a high-fat diet containing 52 fat. In addition to the high-fat feed, 15 mlkg kefir was given to the HFDKefir group via oral gavage. Lipid profile was measured on an autoanalyzer using commercial kits. Leptin, Adiponectin, Resistin and IrisinFNDC5 levels were measured by enzyme-linked immunosorbent assay ELISA using commer-cially available kits. Results: As a result of the experiment, there was no difference between the live weight gains of the groups. Epididymal fat weights in the HFD and HFDKefir groups were found to be statistically significantly higher than the control group. There was no significant difference between the epididymal fat weights of the HFD and HFDKefir groups HDL Cholesterol HDL-C, LDL Cholesterol LDL-C values in the HFD and HFDKefir groups were found to be statistically significantly higher than the control group. No statistically significant difference was detected between the HFDKefir group and the HFD group in terms of HDL-C, LDL-C values. There was no difference between groups in triglyceride values. Adiponectin and IrisinFNDC5 values of the HFDKefir group were found to be statistically significantly lower than the other groups. There was no statistically significant difference between the control group and the HFD group in terms of Adiponectin and IrisinFNDC5 values. There was no significant difference between the groups in Leptin and Resistin values. Conclusions: It was thought that kefir may have metabolic effects through adipokines in the high-fat diet nutrition model, and it would be useful to support this with human studies.

 Amaç: Yağ dokusunun artmasıyla salgılanan proinflamatuar ve antiinflamatuar hormonlardaki dengesizliğin, yağ dokusunda kronik inflamasyona neden olduğu, bunun da obeziteye bağlı metabolik komplikasyonlara neden olduğu düşünülmektedir. Kefir son zamanlarda obeziteyle mücadelede dikkat çeken bir probiyotik türüdür. Bu çalışma, yüksek yağlı diyetle beslenen BALBC fare modelinde kefir tüketiminin lipit profili ve Adiponektin, Leptin, Resistin ve IrisinFNDC5 üzerindeki etkilerini incelemeyi amaçladı. Materyal ve Metod: BALBc suşu erkek fareler; Kontrol grubu n10, yüksek yağlı diyet HFD n10 ve HFDKefir n10 olmak üzere 3 gruba ayrıldılar. Fareler 8 hafta boyunca belirli diyet kalıplarıyla beslendi. Kontrol grubuna standart pelet yem verildi. HFD grubuna 60 yağ içeren yüksek yağlı bir diyet verildi. YYDKefir grubuna yüksek yağlı yemin yanı sıra 15 mlkg kefir oral gavaj yoluyla verildi. Lipid profili, ticari kitler kullanılarak bir otoanalizörde ölçüldü. Leptin, Adiponektin, Resistin ve İrisinFNDC5 seviyeleri, ticari olarak temin edilebilen kitler kullanılarak enzim bağlantılı immünosorbent tahlili ELISA ile ölçüldü. Bulgular: Deneme sonucunda grupların canlı ağırlık artışları arasında fark saptanmadı. HFD ve HFDKefir gruplarında epididimal yağ ağırlıkları kontrol grubuna göre istatistiksel olarak anlamlı düzeyde yüksek bulunmuştur. HFD ve HFDKefir gruplarının epididimal yağ ağırlıkları arasında anlamlı fark yoktu. HFD ve HFDKefir gruplarında total kolesterol, HDL-C, LDL-C değerleri Kontrol grubuna göre istatistiksel olarak anlamlı düzeyde yüksek bulunmuştur. HFDKefir grubu ile HFD grubu arasında Total kolesterol, HDL-K, LDL-K değerleri açısından istatistiksel olarak anlamlı bir fark saptanmadı. Trigliserit değerlerinde gruplar arasında fark yoktu. HFDKefir grubunun adiponektin ve İrisinFNDC5 değerleri diğer gruplara göre istatistiksel olarak anlamlı derecede düşük bulundu. Kontrol grubu ile HFD grubu arasında Adiponektin ve İrisinFNDC5 değerleri açısından istatistiksel olarak anlamlı fark saptanmadı. Leptin ve Resistin değerlerinde gruplar arasında anlamlı fark yoktu. Sonuç: Yüksek yağlı diyet beslenme modelinde kefirin adipokinler aracılığıyla metabolik etkilere sahip olabileceği ve bunun insan çalışmaları ile desteklenmesinin faydalı olacağı düşünüldü.

Makale detayları

Effect of Kefir Consumption on Lipid Metabolism and Adipokine Hormones in BALB/C Mice Fed A High-Fat Diet

Yazarlar:

Seren Orhan | Hülya Çiçek | Mustafa Örkmez | Mehmet Akif Bozdayı | Efsane Yavuz | Davut Sinan Kaplan | Ahmet Sarper Bozkurt

Yayıncı:

Harran University

Ana Başlıklar:

Tıbbi biyokimya - Proteinler | Peptitler ve Proteomik

Dergi:

Harran Üniversitesi Tıp Fakültesi Dergisi Vol.20 Issue 3, pp.678-683

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001298518

pISSN:

1304-9623

High fat diet

Probiotic

Kefir

AdipokinesYüksek yağlı diyet

Probiyotik

Kefir

Adipokinler

Sayı

Makale detayları

Yazarlar: Seren Orhan | Hülya Çiçek | Mustafa Örkmez | Mehmet Akif Bozdayı | Efsane Yavuz | Davut Sinan Kaplan | Ahmet Sarper Bozkurt

Yayıncı: Harran University

Ana Başlıklar: Tıbbi biyokimya - Proteinler | Peptitler ve Proteomik

Dergi: Harran Üniversitesi Tıp Fakültesi Dergisi Vol.20 Issue 3, pp.678-683

Belge Tipi: Makale

Yayın Tarihi: 12/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001298518

pISSN: 1304-9623

Sayı

Yazarlar:

Seren Orhan | Hülya Çiçek | Mustafa Örkmez | Mehmet Akif Bozdayı | Efsane Yavuz | Davut Sinan Kaplan | Ahmet Sarper Bozkurt

Yayıncı:

Harran University

Ana Başlıklar:

Tıbbi biyokimya - Proteinler | Peptitler ve Proteomik

Dergi:

Harran Üniversitesi Tıp Fakültesi Dergisi Vol.20 Issue 3, pp.678-683

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001298518

pISSN:

1304-9623