Mathematical Modelling of Galactooligosaccharides Synthesis: Insights into Aspergillus oryzae Derived β-Galactosidase Kinetics

Kadir Çınar; Hacı Ali Güleç; Gürbüz Güneş

Makale detayları

Mathematical Modelling of Galactooligosaccharides Synthesis: Insights into Aspergillus oryzae Derived β-Galactosidase Kinetics

Yazarlar:

Kadir Çınar | Hacı Ali Güleç | Gürbüz Güneş

Yayıncı:

Tekirdag Namik Kemal University

Ana Başlıklar:

Gıda Biyoteknolojisi | Gıda Teknolojileri | Gıda Bilimleri (Diğer)

Dergi:

Tekirdağ Ziraat Fakültesi Dergisi Vol.22 Issue 2, pp.337-348

Belge Tipi:

Makale

Yayın Tarihi:

5/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001348108

pISSN:

1302-7050

Anahtar kelimeler:

Özet:

 The objective of this study was to adapt experimental data with a mathematical model based on transgalactosylation mechanisms identified in the literature for the synthesis of galactooligosaccharides and lactose hydrolysis. Galactooligosaccharides, synthesized from lactose through β-galactosidase-catalyzed transgalactosylation reactions, are acknowledged as specific bifidogenic factors among prebiotic carbohydrates. Modelling galactooligosaccharide synthesis is challenging due to simultaneous hydrolysis and transferase reactions with identical nucleophiles and substrates. Therefore, researchers tried to simplify the mechanism by ignoring intermediate reactions or inhibitory effects of glucose and galactose. This study explored various kinetic models, considering the complexity of the reaction mechanism and balancing accuracy with feasibility. Experimental data from galactooligosaccharide synthesis using a continuous stirred tank reactor was adapted to kinetic models Models A and B with modifications, incorporating glucose and galactose inhibitions. The COPASI software was used for testing fitness of reaction models. Eight models were evaluated for their suitability, utilizing six replicated experimental datasets under specific reaction conditions, including a temperature of 45C, lactose concentration of 18.25Brix, and enzyme concentration of 10-unit g lactose solution-1. The analysis was conducted using the random research and particle swarm algorithms. Model evaluations revealed that Model B, augmented with glucose and galactose inhibitions, provided the closest alignment between experimental and simulated data. The study underscored the significance of glucose and galactose impacts on galactooligosaccharide synthesis efficiency. The Model B, with addition of glucose and galactose inhibitions, emerged as a valuable tool for predicting galactooligosaccharide yield, contributing to a deeper understanding of A.oryzae-derived β-galactosidase kinetics due to its significant influence on parameters such as productivity, conversion of lactose, and composition of product. The findings will guide optimization strategies for enhanced galactooligosaccharide production efficiency, advancing knowledge in enzymatic galactooligosaccharide synthesis.

 The objective of this study was to adapt experimental data with a mathematical model based on transgalactosylation mechanisms identified in the literature for the synthesis of galactooligosaccharides and lactose hydrolysis. Galactooligosaccharides, synthesized from lactose through β-galactosidase-catalyzed transgalactosylation reactions, are acknowledged as specific bifidogenic factors among prebiotic carbohydrates. Modelling galactooligosaccharide synthesis is challenging due to simultaneous hydrolysis and transferase reactions with identical nucleophiles and substrates. Therefore, researchers tried to simplify the mechanism by ignoring intermediate reactions or inhibitory effects of glucose and galactose. This study explored various kinetic models, considering the complexity of the reaction mechanism and balancing accuracy with feasibility. Experimental data from galactooligosaccharide synthesis using a continuous stirred tank reactor was adapted to kinetic models Models A and B with modifications, incorporating glucose and galactose inhibitions. The COPASI software was used for testing fitness of reaction models. Eight models were evaluated for their suitability, utilizing six replicated experimental datasets under specific reaction conditions, including a temperature of 45C, lactose concentration of 18.25Brix, and enzyme concentration of 10-unit g lactose solution-1. The analysis was conducted using the random research and particle swarm algorithms. Model evaluations revealed that Model B, augmented with glucose and galactose inhibitions, provided the closest alignment between experimental and simulated data. The study underscored the significance of glucose and galactose impacts on galactooligosaccharide synthesis efficiency. The Model B, with addition of glucose and galactose inhibitions, emerged as a valuable tool for predicting galactooligosaccharide yield, contributing to a deeper understanding of A.oryzae-derived β-galactosidase kinetics due to its significant influence on parameters such as productivity, conversion of lactose, and composition of product. The findings will guide optimization strategies for enhanced galactooligosaccharide production efficiency, advancing knowledge in enzymatic galactooligosaccharide synthesis.

 The objective of this study was to adapt experimental data with a mathematical model based on transgalactosylation mechanisms identified in the literature for the synthesis of galactooligosaccharides and lactose hydrolysis. Galactooligosaccharides, synthesized from lactose through β-galactosidase-catalyzed transgalactosylation reactions, are acknowledged as specific bifidogenic factors among prebiotic carbohydrates. Modelling galactooligosaccharide synthesis is challenging due to simultaneous hydrolysis and transferase reactions with identical nucleophiles and substrates. Therefore, researchers tried to simplify the mechanism by ignoring intermediate reactions or inhibitory effects of glucose and galactose. This study explored various kinetic models, considering the complexity of the reaction mechanism and balancing accuracy with feasibility. Experimental data from galactooligosaccharide synthesis using a continuous stirred tank reactor was adapted to kinetic models Models A and B with modifications, incorporating glucose and galactose inhibitions. The COPASI software was used for testing fitness of reaction models. Eight models were evaluated for their suitability, utilizing six replicated experimental datasets under specific reaction conditions, including a temperature of 45C, lactose concentration of 18.25Brix, and enzyme concentration of 10-unit g lactose solution-1. The analysis was conducted using the random research and particle swarm algorithms. Model evaluations revealed that Model B, augmented with glucose and galactose inhibitions, provided the closest alignment between experimental and simulated data. The study underscored the significance of glucose and galactose impacts on galactooligosaccharide synthesis efficiency. The Model B, with addition of glucose and galactose inhibitions, emerged as a valuable tool for predicting galactooligosaccharide yield, contributing to a deeper understanding of A.oryzae-derived β-galactosidase kinetics due to its significant influence on parameters such as productivity, conversion of lactose, and composition of product. The findings will guide optimization strategies for enhanced galactooligosaccharide production efficiency, advancing knowledge in enzymatic galactooligosaccharide synthesis.

 Bu çalışmanın amacı, elde edilen deneysel verileri literatürde tanımlanmış laktoz hidrolizi ve galaktooligosakkaritlerin sentezi için belirlenmiş transgalaktosilasyon mekanizmalarına dayalı matematiksel bir modele uyumlu hale getirmektir. Galaktooligosakkaritler, laktozun β-galaktosidaz katalizli transgalaktosilasyon reaksiyonları aracılığıyla sentezlenen özel bifidojenik faktörler olarak bilinirler. Galaktooligosakkarit sentez mekanizmasının, aynı nükleofillere ve substratlara sahip hidroliz ve transferaz reaksiyonlarının eş zamanlı olarak gerçekleşmesi nedeniyle matematiksel modellemesi zorluklar içermektedir. Bu nedenle araştırmacılar, meydana gelen ara reaksiyonları veya glukoz ve galaktozun inhibe edici etkilerini göz ardı ederek mekanizmayı basitleştirmeye çalıştılar. Bu çalışma, reaksiyon mekanizmasının karmaşıklığını göz önünde bulundurarak doğruluk ile uygulanabilirlik arasında denge sağlamak amacıyla çeşitli kinetik modellerin uygunluğunu değerlendirmektedir. Sürekli karıştırmalı tank reaktörü kullanılarak elde edilen deneysel veriler, glukoz ve galaktoz inhibitörlerini içeren modifikasyonlarla kinetik modellere Model A ve B adapte edilmiştir. COPASI yazılımı, model denklemleri test etmek amacıyla kullanılmıştır. Toplam 8 adet model, 6 tekrarlı deneysel veri setinde sıcaklık:45C, laktoz konsantrasyonu:18.25 Briks ve enzim konsantrasyonu:10 ünite g laktoz çözeltisi-1 uygunluğu test edilmiştir. Çözümleme sırasıyla random research ve particle swarm algoritmaları kullanılarak gerçekleştirilmiştir. Model değerlendirmeleri, glukoz ve galaktoz inhibitörlerini içeren Model Bnin deneysel ve simüle veriler arasında en yakın uyumu sağladığını göstermiştir. Çalışma, glukoz ve galaktozun galaktooligosakkarit sentezi verimliliği üzerindeki inhibitör etkilerinin önemini ortaya koymuştur. Glukoz ve galaktoz inhibitörlerini içeren Model B, galaktooligosakkarit veriminin öngörülmesi ve laktoz dönüşümü, üretkenlik, ürün bileşimi gibi parametreler üzerindeki önemli etkisi nedeniyle A. oryzae kaynaklı β-galaktosidaz kinetiğinin daha iyi anlaşılmasına katkı sağlayacak önemli bir araçtır. Çalışma sonucunda elde edilen bulgular, galaktooligosakkarit üretim verimliliğini artırmak için optimizasyon stratejilerine rehberlik edecek ve enzimatik galaktooligosakkarit sentezi konusunda literatüre katkı sağlayacaktır.

Makale detayları

Mathematical Modelling of Galactooligosaccharides Synthesis: Insights into Aspergillus oryzae Derived β-Galactosidase Kinetics

Yazarlar:

Kadir Çınar | Hacı Ali Güleç | Gürbüz Güneş

Yayıncı:

Tekirdag Namik Kemal University

Ana Başlıklar:

Gıda Biyoteknolojisi | Gıda Teknolojileri | Gıda Bilimleri (Diğer)

Dergi:

Tekirdağ Ziraat Fakültesi Dergisi Vol.22 Issue 2, pp.337-348

Belge Tipi:

Makale

Yayın Tarihi:

5/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001348108

pISSN:

1302-7050

Kinetics

Transgalactosylation

Hydrolysis

Galactooligosaccharides

A. oryzaeKinetik

Transgalaktosilasyon

Hidroliz

Galaktooligosakkaritler

A. oryzae

Sayı

Makale detayları

Yazarlar: Kadir Çınar | Hacı Ali Güleç | Gürbüz Güneş

Yayıncı: Tekirdag Namik Kemal University

Ana Başlıklar: Gıda Biyoteknolojisi | Gıda Teknolojileri | Gıda Bilimleri (Diğer)

Dergi: Tekirdağ Ziraat Fakültesi Dergisi Vol.22 Issue 2, pp.337-348

Belge Tipi: Makale

Yayın Tarihi: 5/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001348108

pISSN: 1302-7050

Sayı

Yazarlar:

Kadir Çınar | Hacı Ali Güleç | Gürbüz Güneş

Yayıncı:

Tekirdag Namik Kemal University

Ana Başlıklar:

Gıda Biyoteknolojisi | Gıda Teknolojileri | Gıda Bilimleri (Diğer)

Dergi:

Tekirdağ Ziraat Fakültesi Dergisi Vol.22 Issue 2, pp.337-348

Belge Tipi:

Makale

Yayın Tarihi:

5/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001348108

pISSN:

1302-7050