PTZ KINDLING MODEL: EVALUATION OF EEG FACTOR AND BIOCHEMISTRY PARAMETERS UNDER THE INFLUENCE OF RAMELTEON

Mukaddes Pala; Ramazan Kozan; Hayrullah Köse; Şenay Görücü Yılmaz

Makale detayları

PTZ KINDLING MODEL: EVALUATION OF EEG FACTOR AND BIOCHEMISTRY PARAMETERS UNDER THE INFLUENCE OF RAMELTEON

Yazarlar:

Mukaddes Pala | Ramazan Kozan | Hayrullah Köse | Şenay Görücü Yılmaz

Yayıncı:

Inonu University

Ana Başlıklar:

Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi:

İnönü Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi Vol.12 Issue 3, pp.843-859

Belge Tipi:

Makale

Yayın Tarihi:

10/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001329332

pISSN:

2147-7892

Anahtar kelimeler:

Özet:

 Many selective synthetic melatonin receptor agonists have anticonvulsantanti-epileptogenic properties. These agonists bind to melatonin receptor 1 MT1 and receptor 2 MT2, causing their activation. Therefore, we evaluated the anticonvulsant effect of Ramelteon RMLT as a melatonin agonist in the PTZ Pentylenetetrazol-kindling model. In the study, 36 male Wistar Albino rats were assessed in 6 groups Sham, PTZ, dimethylsulphoxide DMSO, Valproic acid VPA 150 mgkg  PTZ, RMLT 30 mgkgPTZ, VPARMLTPTZ. Cortical electroencephalography EEG data were recorded for all groups. Seizures were scored according to the Racine scale. Seizure scores and onset times of the first myoclonic movements were compared in EEG traces. Total antioxidant status TAS, total oxidant status TOS, catalase, myeloperoxidase MPO, and Thiol levels were measured in serum samples. Also, Calcineurin CaN, Neuropeptide-Y NPY, Neuron Specific Enolase NSE, and S100B levels were measured in brain tissue samples. There was a significant difference between the PTZ and PTZValproic acidRMLT groups for the onset of the first myoclonic movements and the rate of spikes in the EEG traces in Racines convulsion stages P0.001. Biochemical parameters were not significant between the groups P  0.05. RMLT has anticonvulsant properties. Additionally, the receptor preference of RMLT can be investigated.

 Many selective synthetic melatonin receptor agonists have anticonvulsantanti-epileptogenic properties. These agonists bind to melatonin receptor 1 MT1 and receptor 2 MT2, causing their activation. Therefore, we evaluated the anticonvulsant effect of Ramelteon RMLT as a melatonin agonist in the PTZ Pentylenetetrazol-kindling model. In the study, 36 male Wistar Albino rats were assessed in 6 groups Sham, PTZ, dimethylsulphoxide DMSO, Valproic acid VPA 150 mgkg  PTZ, RMLT 30 mgkgPTZ, VPARMLTPTZ. Cortical electroencephalography EEG data were recorded for all groups. Seizures were scored according to the Racine scale. Seizure scores and onset times of the first myoclonic movements were compared in EEG traces. Total antioxidant status TAS, total oxidant status TOS, catalase, myeloperoxidase MPO, and Thiol levels were measured in serum samples. Also, Calcineurin CaN, Neuropeptide-Y NPY, Neuron Specific Enolase NSE, and S100B levels were measured in brain tissue samples. There was a significant difference between the PTZ and PTZValproic acidRMLT groups for the onset of the first myoclonic movements and the rate of spikes in the EEG traces in Racines convulsion stages P0.001. Biochemical parameters were not significant between the groups P  0.05. RMLT has anticonvulsant properties. Additionally, the receptor preference of RMLT can be investigated.

 Many selective synthetic melatonin receptor agonists have anticonvulsantanti-epileptogenic properties. These agonists bind to melatonin receptor 1 MT1 and receptor 2 MT2, causing their activation. Therefore, we evaluated the anticonvulsant effect of Ramelteon RMLT as a melatonin agonist in the PTZ Pentylenetetrazol-kindling model. In the study, 36 male Wistar Albino rats were assessed in 6 groups Sham, PTZ, dimethylsulphoxide DMSO, Valproic acid VPA 150 mgkg  PTZ, RMLT 30 mgkgPTZ, VPARMLTPTZ. Cortical electroencephalography EEG data were recorded for all groups. Seizures were scored according to the Racine scale. Seizure scores and onset times of the first myoclonic movements were compared in EEG traces. Total antioxidant status TAS, total oxidant status TOS, catalase, myeloperoxidase MPO, and Thiol levels were measured in serum samples. Also, Calcineurin CaN, Neuropeptide-Y NPY, Neuron Specific Enolase NSE, and S100B levels were measured in brain tissue samples. There was a significant difference between the PTZ and PTZValproic acidRMLT groups for the onset of the first myoclonic movements and the rate of spikes in the EEG traces in Racines convulsion stages P0.001. Biochemical parameters were not significant between the groups P  0.05. RMLT has anticonvulsant properties. Additionally, the receptor preference of RMLT can be investigated.

 Pek çok seçici sentetik melatonin reseptörü agonisti, antikonvülsananti-epileptojenik özelliklere sahiptir. Bu agonistler melatonin reseptörü 1 MT1 ve melatonin reseptörü 2ye MT2 bağlanarak onların aktivasyonlarına neden olmaktadır. Bu nedenle, bir melatonin agonisti olarak Ramelteonun RMLT antikonvülsan etkisini PTZ Pentilenetetrazol-kindling modelinde değerlendirdik. Çalışmada 36 adet erkek Wistar Albino sıçan grupta Sham, PTZ, dimetilsülfoksit DMSO, Valproik asit VPA 150 mgkg  PTZ, RMLT 30 mgkgPTZ, VPARMLTPTZ değerlendirildi. Tüm gruplar için kortikal elektroensefalografi EEG verileri kaydedildi. Nöbetler Racine skalasına göre skorlandı. EEG traselerinde nöbet skorları ve ilk miyoklonik hareketlerin başlangıç zamanları karşılaştırıldı. Serum örneklerinde toplam antioksidan durum TAS, toplam oksidan durum TOS, katalaz, miyeloperoksidaz MPO ve Tiyol seviyeleri ölçüldü. Ayrıca beyin dokusu örneklerinde Kalsinörin CaN, Nöropeptid-Y NPY, Nöron Spesifik Enolaz NSE ve S100B seviyeleri ölçüldü. PTZ ve PTZValproik asitRMLT grupları arasında Racinein konvülsiyon evrelerinde ilk miyoklonik hareketlerin başlangıcı ve EEG traselerindeki ani artış oranları açısından anlamlı bir fark vardı P0.001. Biyokimyasal parametreler, gruplar arasında anlamlı değildi P  0.05. RMLT antikonvülsan özelliklere sahiptir. Ek olarak RMLTnin reseptör tercihi de araştırılabilir.

Makale detayları

PTZ KINDLING MODEL: EVALUATION OF EEG FACTOR AND BIOCHEMISTRY PARAMETERS UNDER THE INFLUENCE OF RAMELTEON

Yazarlar:

Mukaddes Pala | Ramazan Kozan | Hayrullah Köse | Şenay Görücü Yılmaz

Yayıncı:

Inonu University

Ana Başlıklar:

Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi:

İnönü Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi Vol.12 Issue 3, pp.843-859

Belge Tipi:

Makale

Yayın Tarihi:

10/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001329332

pISSN:

2147-7892

Anticonvulsant effect

Electroencephalography

Epilepsy

Pentylenetetrazole

RamelteonAntikonvülsan etki

Elektroensefalografi

Epilepsi

Pentilentetrazol

Ramelteon

Sayı

Makale detayları

Yazarlar: Mukaddes Pala | Ramazan Kozan | Hayrullah Köse | Şenay Görücü Yılmaz

Yayıncı: Inonu University

Ana Başlıklar: Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi: İnönü Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi Vol.12 Issue 3, pp.843-859

Belge Tipi: Makale

Yayın Tarihi: 10/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001329332

pISSN: 2147-7892

Sayı

Yazarlar:

Mukaddes Pala | Ramazan Kozan | Hayrullah Köse | Şenay Görücü Yılmaz

Yayıncı:

Inonu University

Ana Başlıklar:

Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi:

İnönü Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi Vol.12 Issue 3, pp.843-859

Belge Tipi:

Makale

Yayın Tarihi:

10/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001329332

pISSN:

2147-7892