Assessment of the Effect of Berberine on Metalloprotease Enzymes Inhibition and Antioxidant Activity: Possible Application in Skin Aging

Shirin Tarbiat

Makale detayları

Assessment of the Effect of Berberine on Metalloprotease Enzymes Inhibition and Antioxidant Activity: Possible Application in Skin Aging

Yazarlar:

Shirin Tarbiat

Yayıncı:

Turkish Chemical Society

Ana Başlıklar:

Reaction Kinetics and Dynamics | Natural Products and Bioactive Compounds | Biologically Active Molecules

Dergi:

Journal of the Turkish Chemical Society Section A: Chemistry Vol.11 Issue 4, pp.1449-1460

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001327162

pISSN:

2149-0120

Anahtar kelimeler:

Özet:

 Skin aging has been defined to enclose both intrinsic and extrinsic aging. Phytochemicals are frequently used for developing skin care formulations and could protect the skins epidermal and dermal layers, consisting mainly of elastin and collagen, from UV radiation. Berberine is an isoquinoline alkaloid and a biologically active component from plant sources. Our objective was to assess Berberines anti-aging capabilities by conducting elastase and collagenase enzyme inhibition and kinetic studies and to also evaluating its antioxidant capacity with three different methods. Furthermore, heat stability, pH and sun protection factor SPF of the formulated cream containing 1.5 berberine was evaluated. The elastase and collagenase IC50 values of berberine were estimated to be 47.54 and, 22.16 µgmL respectively. Berberine was determined as an un-competitive inhibitor of elastase and collagenase. It scavenged DPPH and ABTS free radicals with IC50 values of 66.81 and 180.5 µgmL respectively. 210.387 mgL of berberine was equivalent in reducing power of 176 mgL of ascorbic acid. SPF and pH value of cream containing berberine was found to be 12.3 and 5.62 respectively. In conclusion, these findings suggest that Berberine is a promising candidate for use as an active ingredient in cosmeceuticals, offering a natural approach to enhance skin health and reduce the visible signs of aging.

 Skin aging has been defined to enclose both intrinsic and extrinsic aging. Phytochemicals are frequently used for developing skin care formulations and could protect the skins epidermal and dermal layers, consisting mainly of elastin and collagen, from UV radiation. Berberine is an isoquinoline alkaloid and a biologically active component from plant sources. Our objective was to assess Berberines anti-aging capabilities by conducting elastase and collagenase enzyme inhibition and kinetic studies and to also evaluating its antioxidant capacity with three different methods. Furthermore, heat stability, pH and sun protection factor SPF of the formulated cream containing 1.5 berberine was evaluated. The elastase and collagenase IC50 values of berberine were estimated to be 47.54 and, 22.16 µgmL respectively. Berberine was determined as an un-competitive inhibitor of elastase and collagenase. It scavenged DPPH and ABTS free radicals with IC50 values of 66.81 and 180.5 µgmL respectively. 210.387 mgL of berberine was equivalent in reducing power of 176 mgL of ascorbic acid. SPF and pH value of cream containing berberine was found to be 12.3 and 5.62 respectively. In conclusion, these findings suggest that Berberine is a promising candidate for use as an active ingredient in cosmeceuticals, offering a natural approach to enhance skin health and reduce the visible signs of aging.

 Skin aging has been defined to enclose both intrinsic and extrinsic aging. Phytochemicals are frequently used for developing skin care formulations and could protect the skins epidermal and dermal layers, consisting mainly of elastin and collagen, from UV radiation. Berberine is an isoquinoline alkaloid and a biologically active component from plant sources. Our objective was to assess Berberines anti-aging capabilities by conducting elastase and collagenase enzyme inhibition and kinetic studies and to also evaluating its antioxidant capacity with three different methods. Furthermore, heat stability, pH and sun protection factor SPF of the formulated cream containing 1.5 berberine was evaluated. The elastase and collagenase IC50 values of berberine were estimated to be 47.54 and, 22.16 µgmL respectively. Berberine was determined as an un-competitive inhibitor of elastase and collagenase. It scavenged DPPH and ABTS free radicals with IC50 values of 66.81 and 180.5 µgmL respectively. 210.387 mgL of berberine was equivalent in reducing power of 176 mgL of ascorbic acid. SPF and pH value of cream containing berberine was found to be 12.3 and 5.62 respectively. In conclusion, these findings suggest that Berberine is a promising candidate for use as an active ingredient in cosmeceuticals, offering a natural approach to enhance skin health and reduce the visible signs of aging.

 Skin aging has been defined to enclose both intrinsic and extrinsic aging. Phytochemicals are frequently used for developing skin care formulations and could protect the skins epidermal and dermal layers, consisting mainly of elastin and collagen, from UV radiation. Berberine is an isoquinoline alkaloid and a biologically active component from plant sources. Our objective was to assess Berberines anti-aging capabilities by conducting elastase and collagenase enzyme inhibition and kinetic studies and to also evaluating its antioxidant capacity with three different methods. Furthermore, heat stability, pH and sun protection factor SPF of the formulated cream containing 1.5 berberine was evaluated. The elastase and collagenase IC50 values of berberine were estimated to be 47.54 and, 22.16 µgmL respectively. Berberine was determined as an un-competitive inhibitor of elastase and collagenase. It scavenged DPPH and ABTS free radicals with IC50 values of 66.81 and 180.5 µgmL respectively. 210.387 mgL of berberine was equivalent in reducing power of 176 mgL of ascorbic acid. SPF and pH value of cream containing berberine was found to be 12.3 and 5.62 respectively. In conclusion, these findings suggest that Berberine is a promising candidate for use as an active ingredient in cosmeceuticals, offering a natural approach to enhance skin health and reduce the visible signs of aging.

Makale detayları

Assessment of the Effect of Berberine on Metalloprotease Enzymes Inhibition and Antioxidant Activity: Possible Application in Skin Aging

Yazarlar:

Shirin Tarbiat

Yayıncı:

Turkish Chemical Society

Ana Başlıklar:

Reaction Kinetics and Dynamics | Natural Products and Bioactive Compounds | Biologically Active Molecules

Dergi:

Journal of the Turkish Chemical Society Section A: Chemistry Vol.11 Issue 4, pp.1449-1460

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001327162

pISSN:

2149-0120

Skin aging

Berberine

Collagenase

Elastase

Sayı

Makale detayları

Assessment of the Effect of Berberine on Metalloprotease Enzymes Inhibition and Antioxidant Activity: Possible Application in Skin Aging

Yazarlar: Shirin Tarbiat

Yayıncı: Turkish Chemical Society

Ana Başlıklar: Reaction Kinetics and Dynamics | Natural Products and Bioactive Compounds | Biologically Active Molecules

Dergi: Journal of the Turkish Chemical Society Section A: Chemistry Vol.11 Issue 4, pp.1449-1460

Belge Tipi: Makale

Yayın Tarihi: 3/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001327162

pISSN: 2149-0120

Skin aging

Berberine

Collagenase

Elastase

Sayı

Yazarlar:

Shirin Tarbiat

Yayıncı:

Turkish Chemical Society

Ana Başlıklar:

Reaction Kinetics and Dynamics | Natural Products and Bioactive Compounds | Biologically Active Molecules

Dergi:

Journal of the Turkish Chemical Society Section A: Chemistry Vol.11 Issue 4, pp.1449-1460

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001327162

pISSN:

2149-0120