THE EFFECT OF COENZYME Q10 ADMINISTRATION ON THE EXPRESSION OF Gdnf, Plzf, Sox3, Thy1 GENES IN RATS WITH HYPOTHYROIDISM

Hatice Nur Şeflek; Elif Gülbahçe Mutlu; Gökhan Cüce; Sabiha Serpil Kalkan

Makale detayları

THE EFFECT OF COENZYME Q10 ADMINISTRATION ON THE EXPRESSION OF Gdnf, Plzf, Sox3, Thy1 GENES IN RATS WITH HYPOTHYROIDISM

Yazarlar:

Hatice Nur Şeflek | Elif Gülbahçe Mutlu | Gökhan Cüce | Sabiha Serpil Kalkan

Yayıncı:

Kirikkale University

Ana Başlıklar:

Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi:

Kırıkkale Üniversitesi Tıp Fakültesi Dergisi Vol.26 Issue 2, pp.129-135

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001325254

pISSN:

2148-9645

Anahtar kelimeler:

Özet:

 Objective: Hypothyroidism is a problem of deficient production of thyroid hormones. These hormones regulate metabolism. Therefore, the current health status of the person is adversely affected due to thyroid hormone deficiency. Coenzyme Q10 CoQ10 is a vitamin-like substance with strong antioxidant properties. The aim of this scientific study is to investigate the effects of CoQ10 on hypothyroidism at sera level with important biomarkers glial cell line-derived neurotrophic factor GDNF, promyelocytic leukaemia zinc finger protein PLZF, high mobility group box transcription factor 3 SOX3 and thymocyte differentiation antigen 1 THY1. Material and Methods: Four experimental animal groups were formed: Control group n:7; hypothyroidism group n:7; CoQ10 group n:7; hypothyroidism  CoQ10 group n:7. On the thirty-first day, sera of the animals were collected and Gdnf, Plzf, Sox3, Thy1 expression levels were analyzed in the blood. Results: Significant results occurred in all four biomarkers. As a final result, both hypothyroid pathology was associated with all biomarkers, and CoQ10 positively affected hypothyroidism. Conclusion: The effect of coenzyme Q10 on gene expression levels of Plzf, Gdnf, Thy1, Sox3 at sera level in rats with experimental hypothyroidism was shown by molecular analyses. Coenzyme Q10 regulates sera gene expression levels during treatment.

 Objective: Hypothyroidism is a problem of deficient production of thyroid hormones. These hormones regulate metabolism. Therefore, the current health status of the person is adversely affected due to thyroid hormone deficiency. Coenzyme Q10 CoQ10 is a vitamin-like substance with strong antioxidant properties. The aim of this scientific study is to investigate the effects of CoQ10 on hypothyroidism at sera level with important biomarkers glial cell line-derived neurotrophic factor GDNF, promyelocytic leukaemia zinc finger protein PLZF, high mobility group box transcription factor 3 SOX3 and thymocyte differentiation antigen 1 THY1. Material and Methods: Four experimental animal groups were formed: Control group n:7; hypothyroidism group n:7; CoQ10 group n:7; hypothyroidism  CoQ10 group n:7. On the thirty-first day, sera of the animals were collected and Gdnf, Plzf, Sox3, Thy1 expression levels were analyzed in the blood. Results: Significant results occurred in all four biomarkers. As a final result, both hypothyroid pathology was associated with all biomarkers, and CoQ10 positively affected hypothyroidism. Conclusion: The effect of coenzyme Q10 on gene expression levels of Plzf, Gdnf, Thy1, Sox3 at sera level in rats with experimental hypothyroidism was shown by molecular analyses. Coenzyme Q10 regulates sera gene expression levels during treatment.

 Objective: Hypothyroidism is a problem of deficient production of thyroid hormones. These hormones regulate metabolism. Therefore, the current health status of the person is adversely affected due to thyroid hormone deficiency. Coenzyme Q10 CoQ10 is a vitamin-like substance with strong antioxidant properties. The aim of this scientific study is to investigate the effects of CoQ10 on hypothyroidism at sera level with important biomarkers glial cell line-derived neurotrophic factor GDNF, promyelocytic leukaemia zinc finger protein PLZF, high mobility group box transcription factor 3 SOX3 and thymocyte differentiation antigen 1 THY1. Material and Methods: Four experimental animal groups were formed: Control group n:7; hypothyroidism group n:7; CoQ10 group n:7; hypothyroidism  CoQ10 group n:7. On the thirty-first day, sera of the animals were collected and Gdnf, Plzf, Sox3, Thy1 expression levels were analyzed in the blood. Results: Significant results occurred in all four biomarkers. As a final result, both hypothyroid pathology was associated with all biomarkers, and CoQ10 positively affected hypothyroidism. Conclusion: The effect of coenzyme Q10 on gene expression levels of Plzf, Gdnf, Thy1, Sox3 at sera level in rats with experimental hypothyroidism was shown by molecular analyses. Coenzyme Q10 regulates sera gene expression levels during treatment.

 Amaç: Hipotiroidizm, tiroid hormonlarının eksik üretilmesi sorunudur. Bu hormonlar metabolizmayı düzenler. Dolayısıyla tiroid hormon eksikliğine bağlı olarak kişinin mevcut sağlık durumu olumsuz etkilenir. Koenzim Q10 CoQ10 güçlü antioksidan özelliklere sahip vitamin benzeri bir maddedir. Planlamış olduğumuz bu bilimsel çalışma, CoQ10un hipotiroidizm üzerine etkilerini, önemli biyobelirteçler olan glial hücre kaynaklı nörotrofik faktör GDNF, promyelositik lösemi çinko parmağı PLZF, high mobility group box transcription factor 3 SOX3 ve timosit farklılaşma antijeni 1 THY1 ile serum düzeyinde araştırmaktır. Gereç ve Yöntemler: Dört deney hayvanı grubu oluşturuldu: Kontrol grubu n:7; hipotiroidi grubu n:7; CoQ10 grubu n:7; hipotiroidi  CoQ10 grubu n:7. Otuz birinci günde hayvanların serumları alındı ve kanda Gdnf, Plzf, Sox3, Thy1 ekspresyon düzeyleri analiz edildi. Bulgular: Dört biyobelirteçte de anlamlı sonuçlar elde edildi. Nihai sonuç olarak, hem hipotiroid patolojisi tüm bu biyobelirteçlerle ilişkilendirildi hem de CoQ10un hipotiroidizmi olumlu yönde etkilemiştir. Sonuç: Deneysel hipotiroidizmli sıçanlarda koenzim Q10un serum düzeyinde Plzf, Gdnf, Thy1, Sox3ün gen ifade düzeyinde etkisi moleküler analizlerle gösterilmiştir. Koenzim Q10, tedavi sırasında serum gen ifade seviyelerini düzenlemektedir.

Makale detayları

THE EFFECT OF COENZYME Q10 ADMINISTRATION ON THE EXPRESSION OF Gdnf, Plzf, Sox3, Thy1 GENES IN RATS WITH HYPOTHYROIDISM

Yazarlar:

Hatice Nur Şeflek | Elif Gülbahçe Mutlu | Gökhan Cüce | Sabiha Serpil Kalkan

Yayıncı:

Kirikkale University

Ana Başlıklar:

Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi:

Kırıkkale Üniversitesi Tıp Fakültesi Dergisi Vol.26 Issue 2, pp.129-135

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001325254

pISSN:

2148-9645

Gene expression

hypothyroidism

coenzymes

metabolismGen ekspresyonu

hipotiroidizm

koenzimler

metabolizma

Sayı

Makale detayları

Yazarlar: Hatice Nur Şeflek | Elif Gülbahçe Mutlu | Gökhan Cüce | Sabiha Serpil Kalkan

Yayıncı: Kirikkale University

Ana Başlıklar: Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi: Kırıkkale Üniversitesi Tıp Fakültesi Dergisi Vol.26 Issue 2, pp.129-135

Belge Tipi: Makale

Yayın Tarihi: 8/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001325254

pISSN: 2148-9645

Sayı

Yazarlar:

Hatice Nur Şeflek | Elif Gülbahçe Mutlu | Gökhan Cüce | Sabiha Serpil Kalkan

Yayıncı:

Kirikkale University

Ana Başlıklar:

Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi:

Kırıkkale Üniversitesi Tıp Fakültesi Dergisi Vol.26 Issue 2, pp.129-135

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001325254

pISSN:

2148-9645