Dual magnetic field therapy mitigates LPS-induced pancreatic injury by preserving islet structure and endocrine function in rats

Şenay Topsakal; Öznur Kolay; Halil Aşçı; Özlem Özmen

Makale detayları

Dual magnetic field therapy mitigates LPS-induced pancreatic injury by preserving islet structure and endocrine function in rats

Yazarlar:

Şenay Topsakal | Öznur Kolay | Halil Aşçı | Özlem Özmen

Yayıncı:

Pamukkale University

Ana Başlıklar:

Endokrinoloji

Dergi:

Pamukkale Tıp Dergisi Vol.18 Issue 4, pp.839-849

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001341009

pISSN:

1308-0865

Anahtar kelimeler:

Özet:

 Purpose: Sepsis is a life-threatening systemic inflammatory response that can lead to multiorgan failure, including pancreatic dysfunction. This study aimed to investigate the potential protective effects of radiofrequency RF and pulsed magnetic field PM therapies against lipopolysaccharide LPS-induced pancreatic injury in rats. Materials and methods: Forty female Wistar albino rats were randomly assigned to five groups n8 per group: Control, LPS, LPS  RF, LPS  PM, and LPS  PM  RF. LPS was administered intraperitoneally 5 mgkg, followed by RF 30 minutes andor PM 3 hours exposure. Pancreatic tissues were examined histopathologically and immunohistochemically for insulin, glucagon, and amylin expression. Results: LPS administration resulted in significant histopathological damage, including hyperemia, edema, and inflammatory infiltration, as well as reduced insulin and amylin immunopositivity and increased glucagon expression in islets. Both RF and PM treatments alleviated these changes, with the combined PMRF application showing the most prominent protective effects, restoring normal histological architecture and hormonal profiles. Conclusion: RF and PM therapies, particularly in combination, exhibit significant protective effects against sepsis-induced pancreatic injury by mitigating inflammation and preserving islet cell function. These findings highlight a novel non-pharmacological approach for supporting pancreatic health in inflammatory conditions.

 Purpose: Sepsis is a life-threatening systemic inflammatory response that can lead to multiorgan failure, including pancreatic dysfunction. This study aimed to investigate the potential protective effects of radiofrequency RF and pulsed magnetic field PM therapies against lipopolysaccharide LPS-induced pancreatic injury in rats. Materials and methods: Forty female Wistar albino rats were randomly assigned to five groups n8 per group: Control, LPS, LPS  RF, LPS  PM, and LPS  PM  RF. LPS was administered intraperitoneally 5 mgkg, followed by RF 30 minutes andor PM 3 hours exposure. Pancreatic tissues were examined histopathologically and immunohistochemically for insulin, glucagon, and amylin expression. Results: LPS administration resulted in significant histopathological damage, including hyperemia, edema, and inflammatory infiltration, as well as reduced insulin and amylin immunopositivity and increased glucagon expression in islets. Both RF and PM treatments alleviated these changes, with the combined PMRF application showing the most prominent protective effects, restoring normal histological architecture and hormonal profiles. Conclusion: RF and PM therapies, particularly in combination, exhibit significant protective effects against sepsis-induced pancreatic injury by mitigating inflammation and preserving islet cell function. These findings highlight a novel non-pharmacological approach for supporting pancreatic health in inflammatory conditions.

 Purpose: Sepsis is a life-threatening systemic inflammatory response that can lead to multiorgan failure, including pancreatic dysfunction. This study aimed to investigate the potential protective effects of radiofrequency RF and pulsed magnetic field PM therapies against lipopolysaccharide LPS-induced pancreatic injury in rats. Materials and methods: Forty female Wistar albino rats were randomly assigned to five groups n8 per group: Control, LPS, LPS  RF, LPS  PM, and LPS  PM  RF. LPS was administered intraperitoneally 5 mgkg, followed by RF 30 minutes andor PM 3 hours exposure. Pancreatic tissues were examined histopathologically and immunohistochemically for insulin, glucagon, and amylin expression. Results: LPS administration resulted in significant histopathological damage, including hyperemia, edema, and inflammatory infiltration, as well as reduced insulin and amylin immunopositivity and increased glucagon expression in islets. Both RF and PM treatments alleviated these changes, with the combined PMRF application showing the most prominent protective effects, restoring normal histological architecture and hormonal profiles. Conclusion: RF and PM therapies, particularly in combination, exhibit significant protective effects against sepsis-induced pancreatic injury by mitigating inflammation and preserving islet cell function. These findings highlight a novel non-pharmacological approach for supporting pancreatic health in inflammatory conditions.

 Amaç: Sepsis, pankreas disfonksiyonu da dahil olmak üzere çoklu organ yetmezliğine yol açabilen, yaşamı tehdit eden sistemik bir inflamatuar yanıttır. Bu çalışmanın amacı, lipopolisakkarit LPS ile indüklenen pankreas hasarı üzerinde radyo frekans RF ve darbeli manyetik alan PM tedavilerinin koruyucu etkilerini değerlendirmektir. Gereç ve yöntem: Kırk dişi Wistar albino rat Kontrol, LPS, LPSRF, LPSPM ve LPSPMRF olmak üzere rastgele beş gruba ayrıldı n8. LPS, intraperitoneal olarak 5 mgkg uygulandı, ardından RF 30 dakika veveya PM 3 saat uygulamaları yapıldı. Pankreas dokuları histopatolojik olarak incelendi ve immünohistokimyasal olarak insülin, glukagon ve amilin ekspresyonları değerlendirildi. Bulgular: LPS uygulaması sonucunda pankreaslarda hiperemi, ödem ve inflamatuar infiltrasyon gibi belirgin histopatolojik hasarlar gözlendi. Ayrıca adacık hücrelerinde insülin ve amilin ekspresyonları azaldığı, glukagon ekspresyonu ise arttığı dikkati çekti. Hem RF hem de PM tedavilerinin bu değişiklikleri hafiflettiği saptandı. Özellikle PMRF kombinasyonu, normal histolojik yapının ve hormonal expresyonların en belirgin şekilde düzeltilmesini sağladı. Sonuç: RF ve PM tedavileri, özellikle birlikte uygulandıklarında, sepsise bağlı pankreas hasarına karşı önemli koruyucu etkiler göstermektedir. Bu etkiler, inflamasyonu azaltma ve adacık hücre fonksiyonlarını koruma yoluyla gerçekleşmektedir. Bulgular, inflamatuar durumlarda pankreas sağlığını desteklemek için farmakolojik olmayan yeni bir yaklaşımı ortaya koymaktadır.

Makale detayları

Dual magnetic field therapy mitigates LPS-induced pancreatic injury by preserving islet structure and endocrine function in rats

Yazarlar:

Şenay Topsakal | Öznur Kolay | Halil Aşçı | Özlem Özmen

Yayıncı:

Pamukkale University

Ana Başlıklar:

Endokrinoloji

Dergi:

Pamukkale Tıp Dergisi Vol.18 Issue 4, pp.839-849

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001341009

pISSN:

1308-0865

Sepsis

pancreas

radiofrequency

pulsed magnetic field

LPSSepsis

pankreas

radyo frekans

darbeli manyetik alan

LPS

Sayı

Makale detayları

Yazarlar: Şenay Topsakal | Öznur Kolay | Halil Aşçı | Özlem Özmen

Yayıncı: Pamukkale University

Ana Başlıklar: Endokrinoloji

Dergi: Pamukkale Tıp Dergisi Vol.18 Issue 4, pp.839-849

Belge Tipi: Makale

Yayın Tarihi: 1/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001341009

pISSN: 1308-0865

Sayı

Yazarlar:

Şenay Topsakal | Öznur Kolay | Halil Aşçı | Özlem Özmen

Yayıncı:

Pamukkale University

Ana Başlıklar:

Endokrinoloji

Dergi:

Pamukkale Tıp Dergisi Vol.18 Issue 4, pp.839-849

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001341009

pISSN:

1308-0865