Does melatonin alleviate cold preservation injury of the liver?

Ömer GÜNAL; Abdurrahman COŞKUN; Arif ASLANER; Ümran YILDIRIM

Makale detayları

Does melatonin alleviate cold preservation injury of the liver?

Yazarlar:

Ömer GÜNAL | Abdurrahman COŞKUN | Arif ASLANER | Ümran YILDIRIM

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2010 Vol.40 Issue 3, pp.465-470

Belge Tipi:

Makale

Yayın Tarihi:

2/2010

Belge Dili:

Türkçe

Document ID (Org No.):

2000755101

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

To investigate the effect of melatonin added to preservation solution on liver cold ischemia.  Materials and methods: Twenty male Wistar albino rats were divided equally into groups treated with University of Wisconsin (UW) solution with and without melatonin. The LDH, AST, and ACP activities of the preservation solutions were determined in samples collected at 2, 24, 36, and 48 h. Tissue malondialdehyde and heat shock protein (HSP 70) levels were also measured and a histological examination was performed at 48 h.   Results: Melatonin prevented enzyme elevation, induced heat shock protein (HSP 70), and decreased lipid peroxidation effectively in comparison to the control group (P < 0.05). The histological examination revealed that UW solution containing melatonin prevented Kupffer cell activation and inflammation significantly (P < 0.05).  Conclusion: Melatonin in preservation solutions such as UW solution may protect tissue effectively from damage during cold ischemia for up to 48 h.

 

To investigate the effect of melatonin added to preservation solution on liver cold ischemia.  Materials and methods: Twenty male Wistar albino rats were divided equally into groups treated with University of Wisconsin (UW) solution with and without melatonin. The LDH, AST, and ACP activities of the preservation solutions were determined in samples collected at 2, 24, 36, and 48 h. Tissue malondialdehyde and heat shock protein (HSP 70) levels were also measured and a histological examination was performed at 48 h.   Results: Melatonin prevented enzyme elevation, induced heat shock protein (HSP 70), and decreased lipid peroxidation effectively in comparison to the control group (P < 0.05). The histological examination revealed that UW solution containing melatonin prevented Kupffer cell activation and inflammation significantly (P < 0.05).  Conclusion: Melatonin in preservation solutions such as UW solution may protect tissue effectively from damage during cold ischemia for up to 48 h.

Makale detayları

Does melatonin alleviate cold preservation injury of the liver?

Yazarlar:

Ömer GÜNAL | Abdurrahman COŞKUN | Arif ASLANER | Ümran YILDIRIM

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2010 Vol.40 Issue 3, pp.465-470

Belge Tipi:

Makale

Yayın Tarihi:

2/2010

Belge Dili:

Türkçe

Document ID (Org No.):

2000755101

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Key words: Antioxidant

cold preservation

heat shock protein

liver

melatonin

University of Wisconsin solution

Sayı

Makale detayları

Does melatonin alleviate cold preservation injury of the liver?

Yazarlar: Ömer GÜNAL | Abdurrahman COŞKUN | Arif ASLANER | Ümran YILDIRIM

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2010 Vol.40 Issue 3, pp.465-470

Belge Tipi: Makale

Yayın Tarihi: 2/2010

Belge Dili: Türkçe

Document ID (Org No.): 2000755101

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Key words: Antioxidant

cold preservation

heat shock protein

liver

melatonin

University of Wisconsin solution

Sayı

Yazarlar:

Ömer GÜNAL | Abdurrahman COŞKUN | Arif ASLANER | Ümran YILDIRIM

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2010 Vol.40 Issue 3, pp.465-470

Belge Tipi:

Makale

Yayın Tarihi:

2/2010

Belge Dili:

Türkçe

Document ID (Org No.):

2000755101

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144