Isolation and identification of Podospora flexuosa (syn. Cladorrhinum flexuosum), a potential biocontrol agent detected in sugar beet cultivation areas in Türkiye

Meltem Avan; Rıza Kaya

Makale detayları

Isolation and identification of Podospora flexuosa (syn. Cladorrhinum flexuosum), a potential biocontrol agent detected in sugar beet cultivation areas in Türkiye

Yazarlar:

Meltem Avan | Rıza Kaya

Yayıncı:

Hatay Mustafa Kemal University

Ana Başlıklar:

Bitki Koruma (Diğer)

Dergi:

Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi Vol.29 Issue 1, pp.242-249

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001313088

pISSN:

2667-7733

Anahtar kelimeler:

Özet:

 Sugar beet Beta vulgaris L. is widely cultivated in Konya province of Türkiye and about one third of Türkiyes sugar beet production is obtained from this province. As a result of the isolations made from plants showing severe root rot and desiccation symptoms in sugar beet fields in the region in 2015-2017, a new endophytic fungal isolate was obtained from leaves along with soil and foliar disease agents belonging to different fungal species. The fungal isolate has dull yellow, light olive to light brown, fast growing colony-like, flexible conidiophores and septate branched hyphae. The representative fungal isolate did not cause disease symptoms on host plant leaves. Molecular identification of the fungal isolate was carried out using primers specific to the ITS gene region, and it was identified as Podospora flexuosa syn. Cladorrhinum flexuosum based on morphological and molecular characteristics. Since P. flexuosa is reported to be a biological control agent living as a saprophyte in soil and plant materials, it is thought that the fungal isolate obtained in our study can be used as a potential biocontrol agent in the biological control of plant disease agents in the future. To the best of our knowledge, this fungal species is reported for the first time for Türkiye.

 Sugar beet Beta vulgaris L. is widely cultivated in Konya province of Türkiye and about one third of Türkiyes sugar beet production is obtained from this province. As a result of the isolations made from plants showing severe root rot and desiccation symptoms in sugar beet fields in the region in 2015-2017, a new endophytic fungal isolate was obtained from leaves along with soil and foliar disease agents belonging to different fungal species. The fungal isolate has dull yellow, light olive to light brown, fast growing colony-like, flexible conidiophores and septate branched hyphae. The representative fungal isolate did not cause disease symptoms on host plant leaves. Molecular identification of the fungal isolate was carried out using primers specific to the ITS gene region, and it was identified as Podospora flexuosa syn. Cladorrhinum flexuosum based on morphological and molecular characteristics. Since P. flexuosa is reported to be a biological control agent living as a saprophyte in soil and plant materials, it is thought that the fungal isolate obtained in our study can be used as a potential biocontrol agent in the biological control of plant disease agents in the future. To the best of our knowledge, this fungal species is reported for the first time for Türkiye.

 Sugar beet Beta vulgaris L. is widely cultivated in Konya province of Türkiye and about one third of Türkiyes sugar beet production is obtained from this province. As a result of the isolations made from plants showing severe root rot and desiccation symptoms in sugar beet fields in the region in 2015-2017, a new endophytic fungal isolate was obtained from leaves along with soil and foliar disease agents belonging to different fungal species. The fungal isolate has dull yellow, light olive to light brown, fast growing colony-like, flexible conidiophores and septate branched hyphae. The representative fungal isolate did not cause disease symptoms on host plant leaves. Molecular identification of the fungal isolate was carried out using primers specific to the ITS gene region, and it was identified as Podospora flexuosa syn. Cladorrhinum flexuosum based on morphological and molecular characteristics. Since P. flexuosa is reported to be a biological control agent living as a saprophyte in soil and plant materials, it is thought that the fungal isolate obtained in our study can be used as a potential biocontrol agent in the biological control of plant disease agents in the future. To the best of our knowledge, this fungal species is reported for the first time for Türkiye.

 Şeker pancarı Beta vulgaris L. Türkiyenin Konya ilinde yaygın olarak yetiştirilmekte ve Türkiye şeker pancarı üretiminin yaklaşık üçte biri bu ilden elde edilmektedir. 2015-2017 yıllarında bölgedeki şeker pancarı tarlalarında şiddetli kök çürüklüğü ve kuruma belirtileri gösteren bitkilerden yapılan izolasyonlar sonucunda, farklı fungal türlere ait toprak ve yaprak hastalık etmenleri ile birlikte yapraklardan da yeni bir endofitik fungal izolat elde edilmiştir. Fungal izolat besiyerinde donuk sarı, açık zeytinden açık kahverengiye kadar değişen renklerde, hızlı büyüyen koloni benzeri, esnek konidiyoforlara ve septat dallı hiflere sahiptir. Temsili fungal izolat konukçu bitki yapraklarında hastalık belirtilerine neden olmamıştır. Fungal izolatın moleküler tanımlaması ITS gen bölgesine özgü primerler kullanılarak gerçekleştirilirken, morfolojik ve moleküler özelliklerine göre Podospora flexuosa syn. Cladorrhinum flexuosum olarak tanımlanmıştır. P. flexuosanın toprakta ve bitki materyallerinde saprofit olarak yaşayan bir biyolojik kontrol ajanı olarak bildirildiğinden, çalışmamızda elde edilen fungal izolatın gelecekte bitki hastalık etmenlerinin biyolojik kontrolünde potansiyel bir biyokontrol ajanı olarak kullanılabileceği düünülmektedir. Bildiğimiz kadarıyla, bu fungal tür Türkiye için ilk kez rapor edilmektedir.

Makale detayları

Isolation and identification of Podospora flexuosa (syn. Cladorrhinum flexuosum), a potential biocontrol agent detected in sugar beet cultivation areas in Türkiye

Yazarlar:

Meltem Avan | Rıza Kaya

Yayıncı:

Hatay Mustafa Kemal University

Ana Başlıklar:

Bitki Koruma (Diğer)

Dergi:

Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi Vol.29 Issue 1, pp.242-249

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001313088

pISSN:

2667-7733

Beta vulgaris

Podospora flexuosa

Cladorrhinum flexuosum

Mycobiota

ITS

Pathogenicity

Record

TürkiyeBeta vulgaris

Podospora flexuosa

Cladorrhinum flexuosum

Mikobiyota

ITS

Patojenisite

Kayıt

Türkiye

Sayı

Makale detayları

Yazarlar: Meltem Avan | Rıza Kaya

Yayıncı: Hatay Mustafa Kemal University

Ana Başlıklar: Bitki Koruma (Diğer)

Dergi: Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi Vol.29 Issue 1, pp.242-249

Belge Tipi: Makale

Yayın Tarihi: 8/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001313088

pISSN: 2667-7733

Sayı

Yazarlar:

Meltem Avan | Rıza Kaya

Yayıncı:

Hatay Mustafa Kemal University

Ana Başlıklar:

Bitki Koruma (Diğer)

Dergi:

Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi Vol.29 Issue 1, pp.242-249

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001313088

pISSN:

2667-7733