Nitroglycerin challenge induces lateralized headache in nasociliary  nerve-ligated rats: implications for chronic migraine

Didem Tuba AKÇALI; Ahmet Deniz BELEN; C.avni BABACAN; Hayrunnisa Bolay BELEN

Makale detayları

Nitroglycerin challenge induces lateralized headache in nasociliary nerve-ligated rats: implications for chronic migraine

Yazarlar:

Didem Tuba AKÇALI | Ahmet Deniz BELEN | C.avni BABACAN | Hayrunnisa Bolay BELEN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2017 Vol.47 Issue 2, pp.681-688

Belge Tipi:

Makale

Yayın Tarihi:

3/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000751496

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Özet:

 

Background/aim: Chronic migraine is a common debilitating disease with limited treatment options. We aimed to develop a novel  model for chronic migraine by ligating the nasociliary nerve (NCL) and administering nitroglycerin (NTG) to exacerbate acute  headache attacks.  Materials and methods: Exacerbation of the headache was induced by NTG (10 mg/kg, subcutaneously) administered to male Wistar  rats (n = 36) 14 days following unilateral NCL. Cutaneous and cold allodynia was tested using Von Frey (VF) filaments and acetone,  respectively. Elevated plus maze (EPM) results and c-fos immunoreactivity of TNC were investigated.  Results: NTG administration significantly decreased VF threshold values only in the nasociliary nerve (NCN) territory and the  ipsilateral forepaw (P = 0.0001, P = 0.02). Cold allodynia developed in bilateral NCN territories (P = 0.013). The number/rate of entrance  to open arms in the EPM was significantly decreased in NCN-ligated rats (P = 0.042, P = 0.035). Immunohistochemistry disclosed  significantly increased c-fos-positive neurons in ipsilateral brainstem TNC compared to the contralateral side (brain stem LI ipsilateral  25.4 ± 4.7, contralateral 11.8 ± 1.9, P < 0.05) in chronic NCN-ligated rats exposed to acute NTG.  Conclusion: The presented model provides a valid chronic migraine model relevant to humans, as NTG challenge in chronic NCL rats  generated lateralized headache with cephalic and extracephalic allodynia, altered cold sensitivity, anxiety, and neuronal activation in the  nociceptive laminae of brainstem trigeminal pain nuclei.

Makale detayları

Nitroglycerin challenge induces lateralized headache in nasociliary nerve-ligated rats: implications for chronic migraine

Yazarlar: Didem Tuba AKÇALI | Ahmet Deniz BELEN | C.avni BABACAN | Hayrunnisa Bolay BELEN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2017 Vol.47 Issue 2, pp.681-688

Belge Tipi: Makale

Yayın Tarihi: 3/2017

Belge Dili: Türkçe

Document ID (Org No.): 2000751496

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Sayı

Yazarlar:

Didem Tuba AKÇALI | Ahmet Deniz BELEN | C.avni BABACAN | Hayrunnisa Bolay BELEN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2017 Vol.47 Issue 2, pp.681-688

Belge Tipi:

Makale

Yayın Tarihi:

3/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000751496

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144