Survey of  Dirofilaria immitis  in dogs from Sivas Province in the Central Anatolia Region of Turkey

Ahmet Duran ATAŞ; Kürşat ALTAY; Ahmet ALİM; Erkan ÖZKAN

Makale detayları

Survey of Dirofilaria immitis in dogs from Sivas Province in the Central Anatolia Region of Turkey

Yazarlar:

Ahmet Duran ATAŞ | Kürşat ALTAY | Ahmet ALİM | Erkan ÖZKAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2018 Vol.42 Issue 2, pp.130-134

Belge Tipi:

Makale

Yayın Tarihi:

4/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000764687

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Özet:

 

 
                         In this research, we investigate the prevalence of Dirofilaria immitis infection in dogs using microscopical, serological, and molecular methods from the Central Anatolia Region (Sivas) of Turkey and reveal the associated risk factors. Whole blood and serum samples were obtained from 306 dogs older than 7 months. Polymerase chain reaction (PCR) and the modified Knott's method (Knott) were used for detecting the microfilaria and enzyme-linked immunosorbent assay (ELISA) was applied for the presence of D. immitis antigens. The prevalence of D. immitis in dogs was detected as 2.9% (9/306) by PCR and ELISA and as 1.3% (4/306) by Knott. All Knott-positive samples were also found positive by both PCR and ELISA. According to ELISA and PCR results, no statistically significant difference was found between the prevalence in males (4.2%) and females (1.5%); dogs 0.7?2 years old (2.5%), 3-5 years old (2.2%); and more than 6 years old (7.1%); or stray (3.7%) and owned (1.7%) dogs (P > 0.05). One of the PCR products was selected for sequence analyses of the 16S small subunit rRNA gene and the obtained sequence was submitted to GenBank (KJ183078). The 16S rRNA sequence exhibited 100% identity to other D. immitis sequences available in GenBank (FJ799911, FJ799917, FJ799916). This research provides the first data on D. immitis infections in dogs from Sivas Province by using the combined identification techniques.

Makale detayları

Survey of Dirofilaria immitis in dogs from Sivas Province in the Central Anatolia Region of Turkey

Yazarlar: Ahmet Duran ATAŞ | Kürşat ALTAY | Ahmet ALİM | Erkan ÖZKAN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2018 Vol.42 Issue 2, pp.130-134

Belge Tipi: Makale

Yayın Tarihi: 4/2018

Belge Dili: İngilizce

Document ID (Org No.): 2000764687

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Sayı

Yazarlar:

Ahmet Duran ATAŞ | Kürşat ALTAY | Ahmet ALİM | Erkan ÖZKAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2018 Vol.42 Issue 2, pp.130-134

Belge Tipi:

Makale

Yayın Tarihi:

4/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000764687

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128