Rapid Characterization of Garlic Clones with Locus-Specific DNA Markers

Meryem İPEK; Ahmet İPEK; Philipp W. SIMON

Makale detayları

Rapid Characterization of Garlic Clones with Locus-Specific DNA Markers

Yazarlar:

Meryem İPEK | Ahmet İPEK | Philipp W. SIMON

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2008 Vol.32 Issue 5, pp.357-362

Belge Tipi:

Makale

Yayın Tarihi:

4/2008

Belge Dili:

Türkçe

Document ID (Org No.):

2000808577

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Anahtar kelimeler:

Özet:

 

Maintenance of redundant garlic (Allium sativum L.) accessions is expensive due to the necessity of yearly regenerating garlic accessions in germplasm centers. Therefore, rapid characterization of garlic accessions is important for avoiding duplicated genotypes. For this purpose we developed several locus-specific polymerase chain reaction (PCR)-based DNA markers, and tested them for the characterization of garlic clones that were previously analyzed using amplified fragment length polymorphism (AFLP) and randomly amplified polymorphic DNA (RAPD) markers. Among 25 garlic clones, 4 locus-specific markers generated 24 polymorphic alleles. Garlic accessions within a group determined by AFLP and RAPD analyses generally had the same alleles as the locus-specific markers. In addition, several alleles of these locus-specific markers were associated only with certain AFLP and RAPD groups, which can allow rapid assessment of newly collected garlic accessions; therefore, these locus-specific markers can be used as another tool for the rapid characterization of garlic germplasm collections.

 

Maintenance of redundant garlic (Allium sativum L.) accessions is expensive due to the necessity of yearly regenerating garlic accessions in germplasm centers. Therefore, rapid characterization of garlic accessions is important for avoiding duplicated genotypes. For this purpose we developed several locus-specific polymerase chain reaction (PCR)-based DNA markers, and tested them for the characterization of garlic clones that were previously analyzed using amplified fragment length polymorphism (AFLP) and randomly amplified polymorphic DNA (RAPD) markers. Among 25 garlic clones, 4 locus-specific markers generated 24 polymorphic alleles. Garlic accessions within a group determined by AFLP and RAPD analyses generally had the same alleles as the locus-specific markers. In addition, several alleles of these locus-specific markers were associated only with certain AFLP and RAPD groups, which can allow rapid assessment of newly collected garlic accessions; therefore, these locus-specific markers can be used as another tool for the rapid characterization of garlic germplasm collections.

Makale detayları

Rapid Characterization of Garlic Clones with Locus-Specific DNA Markers

Yazarlar:

Meryem İPEK | Ahmet İPEK | Philipp W. SIMON

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2008 Vol.32 Issue 5, pp.357-362

Belge Tipi:

Makale

Yayın Tarihi:

4/2008

Belge Dili:

Türkçe

Document ID (Org No.):

2000808577

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Allium sativum L.

genetic characterization

DNA markers

germplasm

Sayı

Makale detayları

Rapid Characterization of Garlic Clones with Locus-Specific DNA Markers

Yazarlar: Meryem İPEK | Ahmet İPEK | Philipp W. SIMON

Yayıncı: TUBITAK

Dergi: Turkish Journal of Agriculture and Forestry 2008 Vol.32 Issue 5, pp.357-362

Belge Tipi: Makale

Yayın Tarihi: 4/2008

Belge Dili: Türkçe

Document ID (Org No.): 2000808577

Sıklık: İki Aylık

eISSN: 1303-6173

pISSN: 1300-011X

Allium sativum L.

genetic characterization

DNA markers

germplasm

Sayı

Yazarlar:

Meryem İPEK | Ahmet İPEK | Philipp W. SIMON

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2008 Vol.32 Issue 5, pp.357-362

Belge Tipi:

Makale

Yayın Tarihi:

4/2008

Belge Dili:

Türkçe

Document ID (Org No.):

2000808577

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X