Proteomic Analysis Revealed Underlying Biological Pathways Associated with Hormetic Response of Hormone- Positive Breast Cancer Cell Line Exposed to Low-Dose Flavonoid Mixture

Mete Bora TÜZÜNER; Ayşe Begüm CEVİZ

Makale detayları

Proteomic Analysis Revealed Underlying Biological Pathways Associated with Hormetic Response of Hormone- Positive Breast Cancer Cell Line Exposed to Low-Dose Flavonoid Mixture

Yazarlar:

Mete Bora TÜZÜNER | Ayşe Begüm CEVİZ

Yayıncı:

Istanbul University

Dergi:

European Journal of Biology 2021 Vol.80 Issue 2, pp.145-153

Belge Tipi:

Makale

Yayın Tarihi:

12/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000424340

eISSN:

2618-6144

pISSN:

2602-2575

Özet:

 


Objective: A considerable level of evidence has accumulated about the breast cancer risk-reducing effect of consuming specific flavonoids, through the increasing amount of research and epidemiologic studies. Different flavonoids may have different cellular bioavailability and favor, i.e., the occurrence of a hormetic effect, thus it is important to evaluate breast cancer cells’ response to different doses of flavonoids. This study aims to investigate the alterations of the biological pathways in a hormone-positive (HR+) breast cancer cell line as a resemblance for the most common breast cancer subtype, related to the low-dose exposure of the flavonoids. 

Materials and Methods: Different levels of doses were applied to MCF-7 breast cancer cells. In order to determine cellular proliferation, WST-1 analysis was conducted. The highest proliferation was observed with cell lines exposed to a low-dose flavonoid mixture and these were selected for further analysis. Intracellular protein expression were investigated by peptide analysis on a nano LC-MS/MS platform. A protein-protein interaction network and pathway analysis were conducted for the proteins expressed differently between the groups. 

Results: A total of 214 proteins were identified and 36 proteins with significant alterations (≥1.2-fold change, p≤0.05) were detected. Significant changes were observed in the pathways related to carbon metabolism, amino acid biosynthesis, splicing mechanism, mitochondrial protein import and translation elongation pathways. 

Conclusion: Our study demonstrated that flavonoids can have a hormetic effect which can initially alter metabolic pathways vital for cell proliferation and survival. These pathways may include potential targets for enhancing the anticancer activity of the flavonoids.

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Makale detayları

Proteomic Analysis Revealed Underlying Biological Pathways Associated with Hormetic Response of Hormone- Positive Breast Cancer Cell Line Exposed to Low-Dose Flavonoid Mixture

Yazarlar: Mete Bora TÜZÜNER | Ayşe Begüm CEVİZ

Yayıncı: Istanbul University

Dergi: European Journal of Biology 2021 Vol.80 Issue 2, pp.145-153

Belge Tipi: Makale

Yayın Tarihi: 12/2021

Belge Dili: İngilizce

Document ID (Org No.): 2000424340

eISSN: 2618-6144

pISSN: 2602-2575

Sayı

Yazarlar:

Mete Bora TÜZÜNER | Ayşe Begüm CEVİZ

Yayıncı:

Istanbul University

Dergi:

European Journal of Biology 2021 Vol.80 Issue 2, pp.145-153

Belge Tipi:

Makale

Yayın Tarihi:

12/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000424340

eISSN:

2618-6144

pISSN:

2602-2575