Morphological Evaluation of Ovary in Relation to Recovery, Quality of Oocytes and Steroid  Production in Sheep

Mohamed A.m. ALSAFY; K.h. EL-SHAHAT

Makale detayları

Morphological Evaluation of Ovary in Relation to Recovery, Quality of Oocytes and Steroid Production in Sheep

Yazarlar:

Mohamed A.m. ALSAFY | K.h. EL-SHAHAT

Yayıncı:

Nobel Bilim ve Araştırma Merkezi Limited

Dergi:

Journal of Applied Biological Sciences 2011 Vol.5 Issue 3, pp.27-31

Belge Tipi:

Makale

Yayın Tarihi:

10/2011

Belge Dili:

Türkçe

Document ID (Org No.):

2000831527

Sıklık:

Üç Yıllık

eISSN:

2146-0108

Özet:

 

and estradiol) on the morphology of sheep ovaries, with the view of in vitro recovery, quality, and maturation of oocytes. Fifty  ovarian pairs grouped into right, and left, CL bearing and non-CL -bearing ovaries were used in the current study. The weight,  length, width and thickness of ovaries were recorded. The follicles were classified into 3 groups small (>2mm), medium (2-4mm)  and large (>4mm) follicles. Oocytes were classified according to their morphology into 3 grades COCS (Compact cumulus oocyte  complexes), POCS (Partially invested with less than three layers of cumulus cells), DO (denuded oocyte). The concentration of  progesterone and estradiol 17β in the follicular fluid were estimated. The dimensions of ovaries between right and left ovaries  were not significantly different. However, the ovarian dimensions as well as their weight was significantly (P < 0.05) affected by  presence of CL, being higher in CL bearing ovary than that of non-CL bearing ovary. The average number of large follicles were  significantly (P < 0.05) increased in the right ovary (1.0±0.14) when compared to the left ovary (0.6±0.10). The recovered COCs  number was found to be significantly higher (P < 0.05) in the right than left ovaries (2.0±0.13; 1.2 ±0.09 respectively). A greater  number of vesicular follicles and aspirated COCS were found in non-CL bearing ovary (5.0 ±0.97;1.25±0.09 respectively) than in  CL bearing ovary (3.0±0.67;0.80±0.13 respectively). The CL-absent ovaries provide large numbers as well as high quality of COCs  when compared to CL-present ovaries and that CL-absent ovaries can be used to collect quality COCs for in vitro production of  sheep embryos.

Makale detayları

Morphological Evaluation of Ovary in Relation to Recovery, Quality of Oocytes and Steroid Production in Sheep

Yazarlar: Mohamed A.m. ALSAFY | K.h. EL-SHAHAT

Yayıncı: Nobel Bilim ve Araştırma Merkezi Limited

Dergi: Journal of Applied Biological Sciences 2011 Vol.5 Issue 3, pp.27-31

Belge Tipi: Makale

Yayın Tarihi: 10/2011

Belge Dili: Türkçe

Document ID (Org No.): 2000831527

Sıklık: Üç Yıllık

eISSN: 2146-0108

Sayı

Yazarlar:

Mohamed A.m. ALSAFY | K.h. EL-SHAHAT

Yayıncı:

Nobel Bilim ve Araştırma Merkezi Limited

Dergi:

Journal of Applied Biological Sciences 2011 Vol.5 Issue 3, pp.27-31

Belge Tipi:

Makale

Yayın Tarihi:

10/2011

Belge Dili:

Türkçe

Document ID (Org No.):

2000831527

Sıklık:

Üç Yıllık

eISSN:

2146-0108