Protoplast Isolation in Lupin ( Lupinus mutabilis Sweet): Determination of Optimum Explant Sources and Isolation Conditions

Mehmet BABAOĞLU

Makale detayları

Protoplast Isolation in Lupin ( Lupinus mutabilis Sweet): Determination of Optimum Explant Sources and Isolation Conditions

Yazarlar:

Mehmet BABAOĞLU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2000 Vol.24 Issue 3, pp.177-186

Belge Tipi:

Makale

Yayın Tarihi:

2/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000724638

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X

Özet:

 

Effects of cultural factors on the yield, viability and division of protoplasts were investigated in Lupinus mutabilis Sweet  containing a high protein content as well as a reasonable oil content which may make this species an alternative crop to soybean in  Turkey. Explants from different in vitro seedling parts were evaluated on the suitability of protoplast isolation and viability. Leaf  mesophyll was the most suitable tissue as a protoplast source. Pectinases as well as cellulases were essential for the isolation of  protoplasts. Nine percent (w/v) mannitol was suitable to stabilise osmotic pressure together with low salt concentrations in washing  and isolation solutions while 9% (w/v) glucose gave better results in culture medium. Shoot tip protoplasts exhibited a higher viability  than other protoplast sources. Prolonged viability was observed when protoplasts were cultured in low density media. Techniques  such as nurse cultures and electro-stimulation were ineffective. Sustained division of protoplasts in lupin (all sources) was not  possible. However, techniques applied in this study may help other researchers, especially those studying protoplast culture of  recalcitrant plant species, as well as further studies on this species.

 

Effects of cultural factors on the yield, viability and division of protoplasts were investigated in Lupinus mutabilis Sweet  containing a high protein content as well as a reasonable oil content which may make this species an alternative crop to soybean in  Turkey. Explants from different in vitro seedling parts were evaluated on the suitability of protoplast isolation and viability. Leaf  mesophyll was the most suitable tissue as a protoplast source. Pectinases as well as cellulases were essential for the isolation of  protoplasts. Nine percent (w/v) mannitol was suitable to stabilise osmotic pressure together with low salt concentrations in washing  and isolation solutions while 9% (w/v) glucose gave better results in culture medium. Shoot tip protoplasts exhibited a higher viability  than other protoplast sources. Prolonged viability was observed when protoplasts were cultured in low density media. Techniques  such as nurse cultures and electro-stimulation were ineffective. Sustained division of protoplasts in lupin (all sources) was not  possible. However, techniques applied in this study may help other researchers, especially those studying protoplast culture of  recalcitrant plant species, as well as further studies on this species.

Makale detayları

Protoplast Isolation in Lupin ( Lupinus mutabilis Sweet): Determination of Optimum Explant Sources and Isolation Conditions

Yazarlar: Mehmet BABAOĞLU

Yayıncı: TUBITAK

Dergi: Turkish Journal of Botany 2000 Vol.24 Issue 3, pp.177-186

Belge Tipi: Makale

Yayın Tarihi: 2/2000

Belge Dili: Türkçe

Document ID (Org No.): 2000724638

Sıklık: İki Aylık

eISSN: 1303-6106

pISSN: 1300-008X

Sayı

Yazarlar:

Mehmet BABAOĞLU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2000 Vol.24 Issue 3, pp.177-186

Belge Tipi:

Makale

Yayın Tarihi:

2/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000724638

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X