Wound healing and coagulant activity of crude extract metabolites from fungal endophytes

Fulgence Ntangere Mpenda

Makale detayları

Wound healing and coagulant activity of crude extract metabolites from fungal endophytes

Yazarlar:

Fulgence Ntangere Mpenda

Yayıncı:

İzzet KARA

Ana Başlıklar:

Plant Biotechnology | Microbiology (Other)

Dergi:

International Journal of Secondary Metabolite Vol.11 Issue 4, pp.675-686

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001326695

pISSN:

2148-6905

Anahtar kelimeler:

Özet:

 Bleeding from a wound as a result of physical injury is a life-threatening condition. In pursuing new drug structures, the effect of crude fungal extracts fungal isolated from Jatropha multifida on wound healing and coagulation of mouse whole blood was investigated. Jatropha multifida leaves were sterilized, cut into small segments, and then incubated in Potato Dextrose Agar for seven days. Four isolates were purified and their morphologies were characterized. Identification of isolates was confirmed by a molecular protocol. Two crude extracts from Phlebiopsis gigantea OK021602 and Phyllosticta sp OK021603, which exhibited higher phytochemicals composition, were selected and evaluated using wound excision and coagulation of mouse whole blood, by administering 30 µgmL, 50 µgmL and 70 µgmL crude extracts respectively. The percentage of wound healing in mice was higher p0.05 for the crude extracts of Phlebiopsis gigantea OK021602 as compared to that of Phyllosticta sp OK021603. The highest percentages of wound contraction were 99 at 70 µgmL, and 53 at 70 µgmL for Phlebiopsis gigantea OK021602 and Phyllosticta sp OK021603, respectively as compared to the control group which had 42 wound contraction at day 15 post-treatment. The results of the present study clearly indicate that Jatropha multifida leaves harbor endophytic fungi that produce pharmacologically important bioactive secondary metabolites with wound and hemostatic effects; therefore, further exploration is inevitable, particularly for the purification and identification of specific chemical structures of bioactive compounds.

 Bleeding from a wound as a result of physical injury is a life-threatening condition. In pursuing new drug structures, the effect of crude fungal extracts fungal isolated from Jatropha multifida on wound healing and coagulation of mouse whole blood was investigated. Jatropha multifida leaves were sterilized, cut into small segments, and then incubated in Potato Dextrose Agar for seven days. Four isolates were purified and their morphologies were characterized. Identification of isolates was confirmed by a molecular protocol. Two crude extracts from Phlebiopsis gigantea OK021602 and Phyllosticta sp OK021603, which exhibited higher phytochemicals composition, were selected and evaluated using wound excision and coagulation of mouse whole blood, by administering 30 µgmL, 50 µgmL and 70 µgmL crude extracts respectively. The percentage of wound healing in mice was higher p0.05 for the crude extracts of Phlebiopsis gigantea OK021602 as compared to that of Phyllosticta sp OK021603. The highest percentages of wound contraction were 99 at 70 µgmL, and 53 at 70 µgmL for Phlebiopsis gigantea OK021602 and Phyllosticta sp OK021603, respectively as compared to the control group which had 42 wound contraction at day 15 post-treatment. The results of the present study clearly indicate that Jatropha multifida leaves harbor endophytic fungi that produce pharmacologically important bioactive secondary metabolites with wound and hemostatic effects; therefore, further exploration is inevitable, particularly for the purification and identification of specific chemical structures of bioactive compounds.

 Bleeding from a wound as a result of physical injury is a life-threatening condition. In pursuing new drug structures, the effect of crude fungal extracts fungal isolated from Jatropha multifida on wound healing and coagulation of mouse whole blood was investigated. Jatropha multifida leaves were sterilized, cut into small segments, and then incubated in Potato Dextrose Agar for seven days. Four isolates were purified and their morphologies were characterized. Identification of isolates was confirmed by a molecular protocol. Two crude extracts from Phlebiopsis gigantea OK021602 and Phyllosticta sp OK021603, which exhibited higher phytochemicals composition, were selected and evaluated using wound excision and coagulation of mouse whole blood, by administering 30 µgmL, 50 µgmL and 70 µgmL crude extracts respectively. The percentage of wound healing in mice was higher p0.05 for the crude extracts of Phlebiopsis gigantea OK021602 as compared to that of Phyllosticta sp OK021603. The highest percentages of wound contraction were 99 at 70 µgmL, and 53 at 70 µgmL for Phlebiopsis gigantea OK021602 and Phyllosticta sp OK021603, respectively as compared to the control group which had 42 wound contraction at day 15 post-treatment. The results of the present study clearly indicate that Jatropha multifida leaves harbor endophytic fungi that produce pharmacologically important bioactive secondary metabolites with wound and hemostatic effects; therefore, further exploration is inevitable, particularly for the purification and identification of specific chemical structures of bioactive compounds.

 Bleeding from a wound as a result of physical injury is a life-threatening condition. In pursuing new drug structures, the effect of crude fungal extracts fungal isolated from Jatropha multifida on wound healing and coagulation of mouse whole blood was investigated. Jatropha multifida leaves were sterilized, cut into small segments, and then incubated in Potato Dextrose Agar for seven days. Four isolates were purified and their morphologies were characterized. Identification of isolates was confirmed by a molecular protocol. Two crude extracts from Phlebiopsis gigantea OK021602 and Phyllosticta sp OK021603, which exhibited higher phytochemicals composition, were selected and evaluated using wound excision and coagulation of mouse whole blood, by administering 30 µgmL, 50 µgmL and 70 µgmL crude extracts respectively. The percentage of wound healing in mice was higher p0.05 for the crude extracts of Phlebiopsis gigantea OK021602 as compared to that of Phyllosticta sp OK021603. The highest percentages of wound contraction were 99 at 70 µgmL, and 53 at 70 µgmL for Phlebiopsis gigantea OK021602 and Phyllosticta sp OK021603, respectively as compared to the control group which had 42 wound contraction at day 15 post-treatment. The results of the present study clearly indicate that Jatropha multifida leaves harbor endophytic fungi that produce pharmacologically important bioactive secondary metabolites with wound and hemostatic effects; therefore, further exploration is inevitable, particularly for the purification and identification of specific chemical structures of bioactive compounds.

Makale detayları

Wound healing and coagulant activity of crude extract metabolites from fungal endophytes

Yazarlar:

Fulgence Ntangere Mpenda

Yayıncı:

İzzet KARA

Ana Başlıklar:

Plant Biotechnology | Microbiology (Other)

Dergi:

International Journal of Secondary Metabolite Vol.11 Issue 4, pp.675-686

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001326695

pISSN:

2148-6905

Fungal endophyte

Wound healing

Crude extract

Jatropha multifida

CoagulationFungal endophyte

Wound healing

Crude extract

Jatropha multifida

Coagulation

Sayı

Makale detayları

Yazarlar: Fulgence Ntangere Mpenda

Yayıncı: İzzet KARA

Ana Başlıklar: Plant Biotechnology | Microbiology (Other)

Dergi: International Journal of Secondary Metabolite Vol.11 Issue 4, pp.675-686

Belge Tipi: Makale

Yayın Tarihi: 3/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001326695

pISSN: 2148-6905

Sayı

Yazarlar:

Fulgence Ntangere Mpenda

Yayıncı:

İzzet KARA

Ana Başlıklar:

Plant Biotechnology | Microbiology (Other)

Dergi:

International Journal of Secondary Metabolite Vol.11 Issue 4, pp.675-686

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001326695

pISSN:

2148-6905