Development of HPLC method for phenylethyl resorcinol content determination in whitening cream preparation

N.A.

Makale detayları

Development of HPLC method for phenylethyl resorcinol content determination in whitening cream preparation

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.24 Issue 5, pp.748-764

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347999

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 This experiment aimed to develop and validate an analytical method using HPLC for phenylethyl resorcinol PR content determination in the emulsion-cream formula, consisted five preservative agents methylparaben, ethylparaben, propylparaben, butylparaben, and phenoxyethanol. Measurement was carried out with a UV detector and using a C18 column. Previously, a selectivity test was conducted to distinguish PR from the other whitening agents that were hydroquinone, alpha-arbutin, kojic acid, and vitamin C. The mobile phase system was optimized by using acetonitrile, water, and methanol, in a gradient concentration. The optimum condition was set in the flow rate of 1.0 mLmin, which provided a suitable chromatogram. Linearity in the range of 10.48-73.36 mgmL showed a correlation coefficient r  0.9999 and generated the detection and quantification limit of 0.7183 mgL and 2.3943 mgL, respectively. A robustness test was performed by a variate internal and external factor changes also proved the proper precision. Finally, the validated method was applied as an adequate quantitative analysis to determining phenyl ethyl resorcinol content in the whitening cream preparations.

 This experiment aimed to develop and validate an analytical method using HPLC for phenylethyl resorcinol PR content determination in the emulsion-cream formula, consisted five preservative agents methylparaben, ethylparaben, propylparaben, butylparaben, and phenoxyethanol. Measurement was carried out with a UV detector and using a C18 column. Previously, a selectivity test was conducted to distinguish PR from the other whitening agents that were hydroquinone, alpha-arbutin, kojic acid, and vitamin C. The mobile phase system was optimized by using acetonitrile, water, and methanol, in a gradient concentration. The optimum condition was set in the flow rate of 1.0 mLmin, which provided a suitable chromatogram. Linearity in the range of 10.48-73.36 mgmL showed a correlation coefficient r  0.9999 and generated the detection and quantification limit of 0.7183 mgL and 2.3943 mgL, respectively. A robustness test was performed by a variate internal and external factor changes also proved the proper precision. Finally, the validated method was applied as an adequate quantitative analysis to determining phenyl ethyl resorcinol content in the whitening cream preparations.

 This experiment aimed to develop and validate an analytical method using HPLC for phenylethyl resorcinol PR content determination in the emulsion-cream formula, consisted five preservative agents methylparaben, ethylparaben, propylparaben, butylparaben, and phenoxyethanol. Measurement was carried out with a UV detector and using a C18 column. Previously, a selectivity test was conducted to distinguish PR from the other whitening agents that were hydroquinone, alpha-arbutin, kojic acid, and vitamin C. The mobile phase system was optimized by using acetonitrile, water, and methanol, in a gradient concentration. The optimum condition was set in the flow rate of 1.0 mLmin, which provided a suitable chromatogram. Linearity in the range of 10.48-73.36 mgmL showed a correlation coefficient r  0.9999 and generated the detection and quantification limit of 0.7183 mgL and 2.3943 mgL, respectively. A robustness test was performed by a variate internal and external factor changes also proved the proper precision. Finally, the validated method was applied as an adequate quantitative analysis to determining phenyl ethyl resorcinol content in the whitening cream preparations.

 This experiment aimed to develop and validate an analytical method using HPLC for phenylethyl resorcinol PR content determination in the emulsion-cream formula, consisted five preservative agents methylparaben, ethylparaben, propylparaben, butylparaben, and phenoxyethanol. Measurement was carried out with a UV detector and using a C18 column. Previously, a selectivity test was conducted to distinguish PR from the other whitening agents that were hydroquinone, alpha-arbutin, kojic acid, and vitamin C. The mobile phase system was optimized by using acetonitrile, water, and methanol, in a gradient concentration. The optimum condition was set in the flow rate of 1.0 mLmin, which provided a suitable chromatogram. Linearity in the range of 10.48-73.36 mgmL showed a correlation coefficient r  0.9999 and generated the detection and quantification limit of 0.7183 mgL and 2.3943 mgL, respectively. A robustness test was performed by a variate internal and external factor changes also proved the proper precision. Finally, the validated method was applied as an adequate quantitative analysis to determining phenyl ethyl resorcinol content in the whitening cream preparations.

Makale detayları

Development of HPLC method for phenylethyl resorcinol content determination in whitening cream preparation

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.24 Issue 5, pp.748-764

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347999

pISSN:

2630-6344

: Phenyl ethyl resorcinol

preservatives

whitening cream

HPLC

reverse phase

gradient mobile phase

validation method

Sayı

Makale detayları

Yazarlar: N.A.

Yayıncı: Marmara University

Ana Başlıklar: Pharmacology and Pharmaceutical Sciences (Other)

Dergi: Journal of Research in Pharmacy Vol.24 Issue 5, pp.748-764

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001347999

pISSN: 2630-6344

Sayı

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.24 Issue 5, pp.748-764

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347999

pISSN:

2630-6344