Mutagenesis of Bacillus thuringiensis IAM 12077 for increasing poly (-b-)hydroxybutyrate (PHB) production

Pal ADWITIYA; Prabhu ASHWINI; Arun Kumar AVINASH; Rajagopal BADRI; Dadhe KAJAL; Ponnamma VOMSI; Shivakumar SRIVIDYA

Makale detayları

Mutagenesis of Bacillus thuringiensis IAM 12077 for increasing poly (-b-)hydroxybutyrate (PHB) production

Yazarlar:

Pal ADWITIYA | Prabhu ASHWINI | Arun Kumar AVINASH | Rajagopal BADRI | Dadhe KAJAL | Ponnamma VOMSI | Shivakumar SRIVIDYA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2009 Vol.33 Issue 3, pp.225-230

Belge Tipi:

Makale

Yayın Tarihi:

2/2009

Belge Dili:

Türkçe

Document ID (Org No.):

2000813186

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

A gram-positive bacterium that accumulated PHB was isolated from local garden soil in Bangalore. Based on its morphological and physiological properties, and nucleotide sequence (about 1.5 kb) of its 16S rDNA it was identified as Bacillus thuringiensis IAM 12077. In this study the PHB-producing capacity of putative mutant strains of Bacillus thuringiensis IAM 12077 was evaluated. A Bacillus sp. identified as a Bacillus thuringiensis IAM 12077 strain capable of producing 10%-15% dry cell weight (DCW) PHB when grown in nutrient broth for 48 h was subjected to random mutagenesis [physical (UV) and chemical (acridine orange)]. Among the UV-mutants screened, 19 putative mutants produced more PHB than the parental, while 2 strains produced less. Of these mutants, B8 exhibited promising PHB accumulation (24.68%; 1.54-fold) with more PHB production (1.3 g/l; 5.4-fold) than the parental strain. Chemical mutagenesis yielded putative mutants, of which 6 had a decrease, 3 had no change, and 2 had an increase (B3, C2) in PHB production. While the increase in accumulation (19.69%, 1.43-fold and 22.22%, 1.62-fold, respectively, for B3 and C2) was comparable to the increase shown by the UV mutant (B8), the yields did not concomitantly increase (0.43g/l, 1.13-fold and 0.6g/l, 1.57-fold, respectively).

 

A gram-positive bacterium that accumulated PHB was isolated from local garden soil in Bangalore. Based on its morphological and physiological properties, and nucleotide sequence (about 1.5 kb) of its 16S rDNA it was identified as Bacillus thuringiensis IAM 12077. In this study the PHB-producing capacity of putative mutant strains of Bacillus thuringiensis IAM 12077 was evaluated. A Bacillus sp. identified as a Bacillus thuringiensis IAM 12077 strain capable of producing 10%-15% dry cell weight (DCW) PHB when grown in nutrient broth for 48 h was subjected to random mutagenesis [physical (UV) and chemical (acridine orange)]. Among the UV-mutants screened, 19 putative mutants produced more PHB than the parental, while 2 strains produced less. Of these mutants, B8 exhibited promising PHB accumulation (24.68%; 1.54-fold) with more PHB production (1.3 g/l; 5.4-fold) than the parental strain. Chemical mutagenesis yielded putative mutants, of which 6 had a decrease, 3 had no change, and 2 had an increase (B3, C2) in PHB production. While the increase in accumulation (19.69%, 1.43-fold and 22.22%, 1.62-fold, respectively, for B3 and C2) was comparable to the increase shown by the UV mutant (B8), the yields did not concomitantly increase (0.43g/l, 1.13-fold and 0.6g/l, 1.57-fold, respectively).

Makale detayları

Mutagenesis of Bacillus thuringiensis IAM 12077 for increasing poly (-b-)hydroxybutyrate (PHB) production

Yazarlar:

Pal ADWITIYA | Prabhu ASHWINI | Arun Kumar AVINASH | Rajagopal BADRI | Dadhe KAJAL | Ponnamma VOMSI | Shivakumar SRIVIDYA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2009 Vol.33 Issue 3, pp.225-230

Belge Tipi:

Makale

Yayın Tarihi:

2/2009

Belge Dili:

Türkçe

Document ID (Org No.):

2000813186

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Key words: Bacillus thuringiensis IAM 12077

Poly (-b-) hydroxybutyrate

random mutagenesis

Sayı

Makale detayları

Mutagenesis of Bacillus thuringiensis IAM 12077 for increasing poly (-b-)hydroxybutyrate (PHB) production

Yazarlar: Pal ADWITIYA | Prabhu ASHWINI | Arun Kumar AVINASH | Rajagopal BADRI | Dadhe KAJAL | Ponnamma VOMSI | Shivakumar SRIVIDYA

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2009 Vol.33 Issue 3, pp.225-230

Belge Tipi: Makale

Yayın Tarihi: 2/2009

Belge Dili: Türkçe

Document ID (Org No.): 2000813186

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Key words: Bacillus thuringiensis IAM 12077

Poly (-b-) hydroxybutyrate

random mutagenesis

Sayı

Yazarlar:

Pal ADWITIYA | Prabhu ASHWINI | Arun Kumar AVINASH | Rajagopal BADRI | Dadhe KAJAL | Ponnamma VOMSI | Shivakumar SRIVIDYA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2009 Vol.33 Issue 3, pp.225-230

Belge Tipi:

Makale

Yayın Tarihi:

2/2009

Belge Dili:

Türkçe

Document ID (Org No.):

2000813186

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152