Diagnostic Value of Circulating miRNA-16-5p and miRNA-221-3p in Thyroid Cancer

Esin Oktay; Merve Bıyıklı Alemdar; Bilgin Demir; İbrahim Halil Erdoğdu; Nesibe Kahraman Çetin; İmran Kurt Omurlu; Engin Güney; Mustafa Gökhan Ünsal

Makale detayları

Diagnostic Value of Circulating miRNA-16-5p and miRNA-221-3p in Thyroid Cancer

Yazarlar:

Esin Oktay | Merve Bıyıklı Alemdar | Bilgin Demir | İbrahim Halil Erdoğdu | Nesibe Kahraman Çetin | İmran Kurt Omurlu | Engin Güney | Mustafa Gökhan Ünsal

Yayıncı:

Aydin Adnan Menderes University

Ana Başlıklar:

Clinical Oncology

Dergi:

Meandros Medical And Dental Journal Vol.26 Issue 2, pp.226-231

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001348326

pISSN:

2149-9063

Anahtar kelimeler:

Özet:

 Objective: The aim of this study is to investigate the usability of microRNA16-5P and 221-3P levels as biomarkers in determining thyroid cancers. Material and Methods: Patients who underwent thyroid surgery due to suspicious thyroid nodules were included in the study. A total of 142 patients who agreed to participate in the study had 3-5 cc venous blood taken into EDTA tubes and biochemistry tubes before the operation. In the postoperative period, 68 patients with malignant pathology results and 74 patients with benign pathology results were grouped as the control group. miRNA16-5p and miRNA221-3p levels were measured in serum samples taken from the patients. The levels of miRNA levels in malignant and benign patient groups were analyzed. Results: The miRNA 16-5p and miRNA221-3p levels of malignant patients were statistically significantly lower than those of benign patients p0.001. The analysis showed that miRNA-221-3p and miRNA-16-5p values had diagnostic value in predicting thyroid malignancy. Using a cut-off value of 21.69 for miRNA-221-3p, ROC curve analysis detected 89.7 sensitivity and 71.4 specificity AUC0.779, p0.001. Using a cut-off value of 15.34 for miRNA-16-5p, ROC curve analysis detected 32.3 sensitivity and 100 specificity AUC0.708, p0.001. Conclusion: It was observed that miRNA-221-3p and miRNA-16-5p values had diagnostic value in predicting thyroid malignancy and could be potential biomarkers.

 Objective: The aim of this study is to investigate the usability of microRNA16-5P and 221-3P levels as biomarkers in determining thyroid cancers. Material and Methods: Patients who underwent thyroid surgery due to suspicious thyroid nodules were included in the study. A total of 142 patients who agreed to participate in the study had 3-5 cc venous blood taken into EDTA tubes and biochemistry tubes before the operation. In the postoperative period, 68 patients with malignant pathology results and 74 patients with benign pathology results were grouped as the control group. miRNA16-5p and miRNA221-3p levels were measured in serum samples taken from the patients. The levels of miRNA levels in malignant and benign patient groups were analyzed. Results: The miRNA 16-5p and miRNA221-3p levels of malignant patients were statistically significantly lower than those of benign patients p0.001. The analysis showed that miRNA-221-3p and miRNA-16-5p values had diagnostic value in predicting thyroid malignancy. Using a cut-off value of 21.69 for miRNA-221-3p, ROC curve analysis detected 89.7 sensitivity and 71.4 specificity AUC0.779, p0.001. Using a cut-off value of 15.34 for miRNA-16-5p, ROC curve analysis detected 32.3 sensitivity and 100 specificity AUC0.708, p0.001. Conclusion: It was observed that miRNA-221-3p and miRNA-16-5p values had diagnostic value in predicting thyroid malignancy and could be potential biomarkers.

 Objective: The aim of this study is to investigate the usability of microRNA16-5P and 221-3P levels as biomarkers in determining thyroid cancers. Material and Methods: Patients who underwent thyroid surgery due to suspicious thyroid nodules were included in the study. A total of 142 patients who agreed to participate in the study had 3-5 cc venous blood taken into EDTA tubes and biochemistry tubes before the operation. In the postoperative period, 68 patients with malignant pathology results and 74 patients with benign pathology results were grouped as the control group. miRNA16-5p and miRNA221-3p levels were measured in serum samples taken from the patients. The levels of miRNA levels in malignant and benign patient groups were analyzed. Results: The miRNA 16-5p and miRNA221-3p levels of malignant patients were statistically significantly lower than those of benign patients p0.001. The analysis showed that miRNA-221-3p and miRNA-16-5p values had diagnostic value in predicting thyroid malignancy. Using a cut-off value of 21.69 for miRNA-221-3p, ROC curve analysis detected 89.7 sensitivity and 71.4 specificity AUC0.779, p0.001. Using a cut-off value of 15.34 for miRNA-16-5p, ROC curve analysis detected 32.3 sensitivity and 100 specificity AUC0.708, p0.001. Conclusion: It was observed that miRNA-221-3p and miRNA-16-5p values had diagnostic value in predicting thyroid malignancy and could be potential biomarkers.

 Amaç: Bu çalışmanın amacı tiroid kanserlerini belirlemede mikroRNA16-5P ve 221-3P düzeylerinin biyobelirteç olarak kullanılabilirliğini araştırmaktır. Gereç ve Yöntemler: Şüpheli tiroid nodülü nedeniyle tiroid operasyonu yapılan hastalar çalışmaya alındı. Çalışmaya katılmayı kabul eden toplam 142 hastadan operasyon öncesi EDTAlı tüpe ve biyokimya tüpüne 3-5 cc venöz kanları alındı. Ameliyat sonrası dönemde patoloji sonuçlarına göre patoloji sonucu malign olan 68 hasta ile patoloji sonucu benign olan 74 hasta kontrol grubu olarak gruplandırıldı. Hastalardan alınan serum örneklerinde miRNA-16-5p ve miRNA-221-3p düzeyleri ölçüldü. miRNA düzeylerinin malign ve benign hasta gruplarındaki düzeyleri analiz edildi. Sonuçlar: Malign hastaların miRNA-16-5p ve miRNA-221-3p düzeyleri benign hastalara göre istastistiksel olarak anlamlı derecede daha düşük saptandı p0.001. Yapılan analizde miRNA-221-3p ve miRNA-16-5p değerlerinin tiroid malignitesini öngörmede tanısal değeri olduğu görüldü. miRNA-221-3p için 21.69luk bir kesme değeri kullanılarak, ROC eğrisi analizi 89.7lik sensitivite ve 71.4lik spesifite saptandı AUC0.779, p0.001. miRNA-16-5p için 15.34luk bir kesme değeri kullanılarak, ROC eğrisi analizi 32.3lük sensitivite ve 100lük spesifite saptandı AUC0.708, p0.001. Sonuç: miRNA-221-3p ve miRNA-16-5p değerlerinin tiroid malignitesini öngörmede tanısal değeri olduğu, potansiyel biyobelirteç olabilecekleri görülmüştür.

Makale detayları

Diagnostic Value of Circulating miRNA-16-5p and miRNA-221-3p in Thyroid Cancer

Yazarlar:

Esin Oktay | Merve Bıyıklı Alemdar | Bilgin Demir | İbrahim Halil Erdoğdu | Nesibe Kahraman Çetin | İmran Kurt Omurlu | Engin Güney | Mustafa Gökhan Ünsal

Yayıncı:

Aydin Adnan Menderes University

Ana Başlıklar:

Clinical Oncology

Dergi:

Meandros Medical And Dental Journal Vol.26 Issue 2, pp.226-231

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001348326

pISSN:

2149-9063

miRNA

Biomarkers

Thyroid cancermiRNA

Biyobelirteçler

Tiroid kanseri

Sayı

Makale detayları

Diagnostic Value of Circulating miRNA-16-5p and miRNA-221-3p in Thyroid Cancer

Yazarlar: Esin Oktay | Merve Bıyıklı Alemdar | Bilgin Demir | İbrahim Halil Erdoğdu | Nesibe Kahraman Çetin | İmran Kurt Omurlu | Engin Güney | Mustafa Gökhan Ünsal

Yayıncı: Aydin Adnan Menderes University

Ana Başlıklar: Clinical Oncology

Dergi: Meandros Medical And Dental Journal Vol.26 Issue 2, pp.226-231

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001348326

pISSN: 2149-9063

miRNA

Biomarkers

Thyroid cancermiRNA

Biyobelirteçler

Tiroid kanseri

Sayı

Yazarlar:

Esin Oktay | Merve Bıyıklı Alemdar | Bilgin Demir | İbrahim Halil Erdoğdu | Nesibe Kahraman Çetin | İmran Kurt Omurlu | Engin Güney | Mustafa Gökhan Ünsal

Yayıncı:

Aydin Adnan Menderes University

Ana Başlıklar:

Clinical Oncology

Dergi:

Meandros Medical And Dental Journal Vol.26 Issue 2, pp.226-231

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001348326

pISSN:

2149-9063