Transmission of the Embryonic Stem Cell (ESC)  Genome to Offspring and Sexual Development in  Chimeric Mice From a Male Esc Line

Sezen ARAT

Makale detayları

Transmission of the Embryonic Stem Cell (ESC) Genome to Offspring and Sexual Development in Chimeric Mice From a Male Esc Line

Yazarlar:

Sezen ARAT

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2000 Vol.24 Issue 4, pp.707-716

Belge Tipi:

Makale

Yayın Tarihi:

3/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000813922

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

This study was performed to determine the capability of embryonic stem (ES) cells at late  passage number to form functional germ cells and to orientate the sexual development of chimeric  mice produced from ES cell injection into embryos. When an R1 ES cell line was routinely cultured on  mitotically inactivated feeder cells in culture medium supplemented with leukemia inhibitory factor  (LIF), the colonies maintained an undifferentiated ideal ES cell morphology. When the ES cells were  injected into 45 eight-cell embryos, 38 embryos (84%) combined with ES cells. Twenty-eight percent  of the embryos transferred to recipients developed to term. Thirty percent of offspring born were  chimeric. In these chimeric individuals the contribution of ES cells to the coat was about 70% and 99%  in two male and one female chimeras, respectively. Furthermore, ES cells contributed to the germ line  in the female chimera whereas they did not contribute to the germ line in two male chimeras. This  study showed that female chimeras may result from injected embryos with the male ES cell and may  transmit the ES cell genome to their offspring.

 

This study was performed to determine the capability of embryonic stem (ES) cells at late  passage number to form functional germ cells and to orientate the sexual development of chimeric  mice produced from ES cell injection into embryos. When an R1 ES cell line was routinely cultured on  mitotically inactivated feeder cells in culture medium supplemented with leukemia inhibitory factor  (LIF), the colonies maintained an undifferentiated ideal ES cell morphology. When the ES cells were  injected into 45 eight-cell embryos, 38 embryos (84%) combined with ES cells. Twenty-eight percent  of the embryos transferred to recipients developed to term. Thirty percent of offspring born were  chimeric. In these chimeric individuals the contribution of ES cells to the coat was about 70% and 99%  in two male and one female chimeras, respectively. Furthermore, ES cells contributed to the germ line  in the female chimera whereas they did not contribute to the germ line in two male chimeras. This  study showed that female chimeras may result from injected embryos with the male ES cell and may  transmit the ES cell genome to their offspring.

Makale detayları

Transmission of the Embryonic Stem Cell (ESC) Genome to Offspring and Sexual Development in Chimeric Mice From a Male Esc Line

Yazarlar:

Sezen ARAT

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2000 Vol.24 Issue 4, pp.707-716

Belge Tipi:

Makale

Yayın Tarihi:

3/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000813922

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Mouse

ES cells

chimera

sex conversion

germ cells.

Sayı

Makale detayları

Transmission of the Embryonic Stem Cell (ESC) Genome to Offspring and Sexual Development in Chimeric Mice From a Male Esc Line

Yazarlar: Sezen ARAT

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2000 Vol.24 Issue 4, pp.707-716

Belge Tipi: Makale

Yayın Tarihi: 3/2000

Belge Dili: Türkçe

Document ID (Org No.): 2000813922

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Mouse

ES cells

chimera

sex conversion

germ cells.

Sayı

Yazarlar:

Sezen ARAT

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2000 Vol.24 Issue 4, pp.707-716

Belge Tipi:

Makale

Yayın Tarihi:

3/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000813922

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152