Cytotoxic Effect of Prunus Divaricata Ledeb. Extract on Lung Cancer Cells and Determination of Expression Profiles of Genes in the MAP-Kinase Pathway

İbrahim Seyfettin Çelik; Ashabil Aygan; Mustafa Çiçek; Yusuf Ziya Kocabaş

Makale detayları

Cytotoxic Effect of Prunus Divaricata Ledeb. Extract on Lung Cancer Cells and Determination of Expression Profiles of Genes in the MAP-Kinase Pathway

Yazarlar:

İbrahim Seyfettin Çelik | Ashabil Aygan | Mustafa Çiçek | Yusuf Ziya Kocabaş

Yayıncı:

Sakarya University

Ana Başlıklar:

Structural Biology

Dergi:

Sakarya University Journal of Science Vol.27 Issue 1, pp.181-188

Belge Tipi:

Makale

Yayın Tarihi:

2/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001283972

pISSN:

2147-835X

Anahtar kelimeler:

Özet:

 Cherry plum Prunus divaricata Ledeb. is a diploid fruit tree that belongs to the Rosaceae family. Anticarcinogenic activity of Prunus divaricata Ledeb. methanol extract was examined in lung cancer cell lines A549 and H1299 as well as in healthy cell line Beas-2b in this study. The changes in the expression levels of the most frequently mutated oncogenes in lung cancer, KRAS, EGFR, BRAF, and PIK3CA were investigated as well. In vitro cytotoxicity assays of P. divaricata Ledeb. fruit methanol extract was conducted by measuring the percentage of viable cells spectrophotometrically using the cell viability identification kit CVDK based on the colorimetric method. The AB 7500 Fast RT-PCR device with Sybr Green Master Mix was used to create gene expression profiles, and the Ct values of all genes were assessed by normalizing the Ct value of the housekeeping gene. The methanol extract of P. divaricata Ledeb. fruit was found to have a cytotoxic effect on A549 and H1299 cancer cell lines at 20 mgml, but not on the Beas-2b cell line at these concentrations. By reducing proliferation, low KRAS and PIK3CA gene expression caused the cells to undergo apoptosis. The methanol extract of P. divaricata Ledeb. fruit has been found to inhibit the development of cancerous cells while promoting the proliferation of healthy cells. We believe it does this through the apoptotic pathway by regulating gene expression levels in oncogenes via the MAP-Kinase pathway.

 Cherry plum Prunus divaricata Ledeb. is a diploid fruit tree that belongs to the Rosaceae family. Anticarcinogenic activity of Prunus divaricata Ledeb. methanol extract was examined in lung cancer cell lines A549 and H1299 as well as in healthy cell line Beas-2b in this study. The changes in the expression levels of the most frequently mutated oncogenes in lung cancer, KRAS, EGFR, BRAF, and PIK3CA were investigated as well. In vitro cytotoxicity assays of P. divaricata Ledeb. fruit methanol extract was conducted by measuring the percentage of viable cells spectrophotometrically using the cell viability identification kit CVDK based on the colorimetric method. The AB 7500 Fast RT-PCR device with Sybr Green Master Mix was used to create gene expression profiles, and the Ct values of all genes were assessed by normalizing the Ct value of the housekeeping gene. The methanol extract of P. divaricata Ledeb. fruit was found to have a cytotoxic effect on A549 and H1299 cancer cell lines at 20 mgml, but not on the Beas-2b cell line at these concentrations. By reducing proliferation, low KRAS and PIK3CA gene expression caused the cells to undergo apoptosis. The methanol extract of P. divaricata Ledeb. fruit has been found to inhibit the development of cancerous cells while promoting the proliferation of healthy cells. We believe it does this through the apoptotic pathway by regulating gene expression levels in oncogenes via the MAP-Kinase pathway.

 Cherry plum Prunus divaricata Ledeb. is a diploid fruit tree that belongs to the Rosaceae family. Anticarcinogenic activity of Prunus divaricata Ledeb. methanol extract was examined in lung cancer cell lines A549 and H1299 as well as in healthy cell line Beas-2b in this study. The changes in the expression levels of the most frequently mutated oncogenes in lung cancer, KRAS, EGFR, BRAF, and PIK3CA were investigated as well. In vitro cytotoxicity assays of P. divaricata Ledeb. fruit methanol extract was conducted by measuring the percentage of viable cells spectrophotometrically using the cell viability identification kit CVDK based on the colorimetric method. The AB 7500 Fast RT-PCR device with Sybr Green Master Mix was used to create gene expression profiles, and the Ct values of all genes were assessed by normalizing the Ct value of the housekeeping gene. The methanol extract of P. divaricata Ledeb. fruit was found to have a cytotoxic effect on A549 and H1299 cancer cell lines at 20 mgml, but not on the Beas-2b cell line at these concentrations. By reducing proliferation, low KRAS and PIK3CA gene expression caused the cells to undergo apoptosis. The methanol extract of P. divaricata Ledeb. fruit has been found to inhibit the development of cancerous cells while promoting the proliferation of healthy cells. We believe it does this through the apoptotic pathway by regulating gene expression levels in oncogenes via the MAP-Kinase pathway.

 Cherry plum Prunus divaricata Ledeb. is a diploid fruit tree that belongs to the Rosaceae family. Anticarcinogenic activity of Prunus divaricata Ledeb. methanol extract was examined in lung cancer cell lines A549 and H1299 as well as in healthy cell line Beas-2b in this study. The changes in the expression levels of the most frequently mutated oncogenes in lung cancer, KRAS, EGFR, BRAF, and PIK3CA were investigated as well. In vitro cytotoxicity assays of P. divaricata Ledeb. fruit methanol extract was conducted by measuring the percentage of viable cells spectrophotometrically using the cell viability identification kit CVDK based on the colorimetric method. The AB 7500 Fast RT-PCR device with Sybr Green Master Mix was used to create gene expression profiles, and the Ct values of all genes were assessed by normalizing the Ct value of the housekeeping gene. The methanol extract of P. divaricata Ledeb. fruit was found to have a cytotoxic effect on A549 and H1299 cancer cell lines at 20 mgml, but not on the Beas-2b cell line at these concentrations. By reducing proliferation, low KRAS and PIK3CA gene expression caused the cells to undergo apoptosis. The methanol extract of P. divaricata Ledeb. fruit has been found to inhibit the development of cancerous cells while promoting the proliferation of healthy cells. We believe it does this through the apoptotic pathway by regulating gene expression levels in oncogenes via the MAP-Kinase pathway.

Makale detayları

Cytotoxic Effect of Prunus Divaricata Ledeb. Extract on Lung Cancer Cells and Determination of Expression Profiles of Genes in the MAP-Kinase Pathway

Yazarlar:

İbrahim Seyfettin Çelik | Ashabil Aygan | Mustafa Çiçek | Yusuf Ziya Kocabaş

Yayıncı:

Sakarya University

Ana Başlıklar:

Structural Biology

Dergi:

Sakarya University Journal of Science Vol.27 Issue 1, pp.181-188

Belge Tipi:

Makale

Yayın Tarihi:

2/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001283972

pISSN:

2147-835X

Anticancer

gene expression

P. divaricata subs P. divaricata Ledeb.

Anticancer

gene expression

P. divaricata subsp. divaricata Ledeb. A549

H1299 and Beas-2b

Anticancer

gene expression

P. divaricata subs P. divaricata Ledeb.

Sayı

Makale detayları

Yazarlar: İbrahim Seyfettin Çelik | Ashabil Aygan | Mustafa Çiçek | Yusuf Ziya Kocabaş

Yayıncı: Sakarya University

Ana Başlıklar: Structural Biology

Dergi: Sakarya University Journal of Science Vol.27 Issue 1, pp.181-188

Belge Tipi: Makale

Yayın Tarihi: 2/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001283972

pISSN: 2147-835X

Sayı

Yazarlar:

İbrahim Seyfettin Çelik | Ashabil Aygan | Mustafa Çiçek | Yusuf Ziya Kocabaş

Yayıncı:

Sakarya University

Ana Başlıklar:

Structural Biology

Dergi:

Sakarya University Journal of Science Vol.27 Issue 1, pp.181-188

Belge Tipi:

Makale

Yayın Tarihi:

2/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001283972

pISSN:

2147-835X