Some Properties of Crude Carboxymethyl Cellulase of Aspergillus niger Z10 Wild-Type Strain

Gökhan CORAL

Makale detayları

Some Properties of Crude Carboxymethyl Cellulase of Aspergillus niger Z10 Wild-Type Strain

Yazarlar:

Gökhan CORAL

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2002 Vol.26 Issue 4, pp.209-213

Belge Tipi:

Makale

Yayın Tarihi:

3/2002

Belge Dili:

Türkçe

Document ID (Org No.):

2000813676

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

A carboxymethyl cellulase enzyme was prepared from a wild type strain of Aspergillus niger Z10. Analyses of the enzyme preparation by SDS-PAGE revealed two protein bands showing cellulolytic activity. The molecular weight of these bands was estimated to be around 83,000 and 50,000. The optimum temperature of the enzyme was observed to be around 40 °C. It was found that the enzyme's activity has a broad pH range between 3 to 9 and 41.2% of the original activity was retained after heat treatment at 90 °C for 15 min.

 

A carboxymethyl cellulase enzyme was prepared from a wild type strain of Aspergillus niger Z10. Analyses of the enzyme preparation by SDS-PAGE revealed two protein bands showing cellulolytic activity. The molecular weight of these bands was estimated to be around 83,000 and 50,000. The optimum temperature of the enzyme was observed to be around 40 °C. It was found that the enzyme's activity has a broad pH range between 3 to 9 and 41.2% of the original activity was retained after heat treatment at 90 °C for 15 min.

Makale detayları

Some Properties of Crude Carboxymethyl Cellulase of Aspergillus niger Z10 Wild-Type Strain

Yazarlar:

Gökhan CORAL

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2002 Vol.26 Issue 4, pp.209-213

Belge Tipi:

Makale

Yayın Tarihi:

3/2002

Belge Dili:

Türkçe

Document ID (Org No.):

2000813676

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Aspergillus niger

Carboxymethyl cellulase (CMCase)

Sayı

Makale detayları

Some Properties of Crude Carboxymethyl Cellulase of Aspergillus niger Z10 Wild-Type Strain

Yazarlar: Gökhan CORAL

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2002 Vol.26 Issue 4, pp.209-213

Belge Tipi: Makale

Yayın Tarihi: 3/2002

Belge Dili: Türkçe

Document ID (Org No.): 2000813676

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Aspergillus niger

Carboxymethyl cellulase (CMCase)

Sayı

Yazarlar:

Gökhan CORAL

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2002 Vol.26 Issue 4, pp.209-213

Belge Tipi:

Makale

Yayın Tarihi:

3/2002

Belge Dili:

Türkçe

Document ID (Org No.):

2000813676

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152